PMID- 16231057
OWN - NLM
STAT- MEDLINE
DCOM- 20060209
LR  - 20061115
IS  - 0969-7128 (Print)
IS  - 0969-7128 (Linking)
VI  - 12 Suppl 1
DP  - 2005 Oct
TI  - Purification of adenovirus and adeno-associated virus: comparison of novel 
      membrane-based technology to conventional techniques.
PG  - S62-72
AB  - Adenovirus (Ad) and Adeno-associated virus (AAV) are efficient gene delivery 
      systems; manipulation of the wild-type genome allows their use as vectors for the 
      overexpression of desirable transgenes. Generation and purification of such viral 
      vectors can be labour intensive, costly and require specialized equipment, but a 
      new generation of membrane-mediated ion exchange kits for purification of 
      recombinant virus may facilitate this process. Here, we examine the yields, 
      transgene expression and purity of preparations of Ad and AAV purified using 
      commercially available kits in comparison to other established techniques for 
      purification of recombinant viral vectors. We demonstrate comparable results for 
      Ad and AAV respectively in all parameters investigated, with a substantial 
      reduction in purification time for the kit-based technology. Such approaches are 
      attractive methods for small-scale purification of recombinant Ad and AAV viral 
      vectors.
FAU - Duffy, A M
AU  - Duffy AM
AD  - Department of Medicine and Regenerative Medicine Institute (REMEDI), National 
      University of Ireland, Galway, Ireland.
FAU - O'Doherty, A M
AU  - O'Doherty AM
FAU - O'Brien, T
AU  - O'Brien T
FAU - Strappe, P M
AU  - Strappe PM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Gene Ther
JT  - Gene therapy
JID - 9421525
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - *Adenoviridae
MH  - Biotechnology
MH  - Cell Line
MH  - *Dependovirus
MH  - Free Radical Scavengers
MH  - Gene Transfer Techniques
MH  - Genetic Engineering
MH  - Genetic Vectors/*isolation & purification
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Recombinant Fusion Proteins/isolation & purification
MH  - Staining and Labeling
MH  - Superoxide Dismutase/analysis/genetics
MH  - Transgenes
MH  - Virology/methods
EDAT- 2005/10/19 09:00
MHDA- 2006/02/10 09:00
CRDT- 2005/10/19 09:00
PHST- 2005/10/19 09:00 [pubmed]
PHST- 2006/02/10 09:00 [medline]
PHST- 2005/10/19 09:00 [entrez]
AID - 3302616 [pii]
AID - 10.1038/sj.gt.3302616 [doi]
PST - ppublish
SO  - Gene Ther. 2005 Oct;12 Suppl 1:S62-72. doi: 10.1038/sj.gt.3302616.