PMID- 16232652
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20051101
LR  - 20191026
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 88
IP  - 5
DP  - 1999
TI  - Classification and identification of strains of Lactobacillus brevis based on 
      electrophoretic characterization of D-lactate dehydrogenase: relationship between 
      D-lactate dehydrogenase and beer-spoilage ability.
PG  - 500-6
AB  - The cell-free extracts of 60 strains which were identified phenotypically as 
      being those of Lactobacillus brevis, including 48 isolates from the environment 
      and 12 reference strains, were applied to polyacrylamide gel electrophoresis for 
      extracting their NAD-dependent D- and L-lactate dehydrogenases (LDH). These 
      strains were divided into 5 groups, i.e., Groups A, B, C, D, and E, on the basis 
      of the electrophoretic mobilities of their D-LDH. The strains showed variations 
      in their carbohydrate fermentation patterns. No relationship between the profile 
      of D-LDH and the carbohydrate fermentation pattern was recognized. However, there 
      appeared to be a relationship between the D-LDH profile and the beer-spoilage 
      ability, because 40 out of 44 beer-spoilage strains identified as L. brevis were 
      classified to Group B. We purified D-LDHs from the so-called complete 
      beer-spoilage strain SBC 8002 of LDH Group B and from the non beer-spoilage 
      strains JCM 1059T of LDH Group A and AHU 1508 of LDH Group C. Although the 
      purified D-LDHs had the same molecular weight (84 kDa), each possessed a 
      different optimum pH, optimum temperature, and isoelectric point. The 
      aforementioned parameter values for the enzyme from the so-called complete 
      beer-spoilage strain SBC 8002 of LDH Group B were 10.0, 50 degrees C, and 4.1, 
      respectively; this strain was discriminated from the D-LDHs of the other two non 
      beer-spoilage strains especially by its optimum temperature (50 degrees C).
FAU - Takahashi, T
AU  - Takahashi T
AD  - Brewing Research Laboratories, Sapporo Breweries Ltd., 10 Okatome, Yaizu, 
      Shizuoka 425-0013, Japan.
FAU - Nakakita, Y
AU  - Nakakita Y
FAU - Sugiyama, H
AU  - Sugiyama H
FAU - Shigyo, T
AU  - Shigyo T
FAU - Shinotsuka, K
AU  - Shinotsuka K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
EDAT- 2005/10/20 09:00
MHDA- 2005/10/20 09:01
CRDT- 2005/10/20 09:00
PHST- 1998/07/15 00:00 [received]
PHST- 1999/08/09 00:00 [accepted]
PHST- 1998/07/15 00:00 [received]
PHST- 1999/08/09 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2005/10/20 09:01 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S1389-1723(00)87666-5 [pii]
AID - 10.1016/s1389-1723(00)87666-5 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 1999;88(5):500-6. doi: 10.1016/s1389-1723(00)87666-5.