PMID- 16232968
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20051101
LR  - 20191026
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 91
IP  - 2
DP  - 2001
TI  - Denaturing gradient gel electrophoresis analyses of microbial community from 
      field-scale composter.
PG  - 159-65
AB  - The diversity of microbial community during the decomposition of waste in a 
      field-scale composter (Hazaka system) was investigated by denaturing gradient gel 
      electrophoresis (DGGE). The composter operates at a high temperature through a 
      self-heating system, creating a thermophilic (60-76 degrees C) stage during the 
      initial phase and a mesophilic (45 degrees C) stage towards the later phase of 
      the composting period. The pH of the system (pH 7.75-8.10) did not vary 
      significantly during the process while moisture content was reduced from 48.8% to 
      25.1%. DGGE and 16S rDNA analyses showed that the following genera were found 
      throughout the process: Propionibacterium sp., Methylobacterium sp., Pseudomonas 
      sp., and Bradyrhizobium sp. Different Bacillus spp. thrive at the thermophilic or 
      the mesophilic stage while Clostridium sp. was only found at the initial phase of 
      the process. Staphylococcus sp. and Caulobacter sp. or Brevundimonas sp. existed 
      during the later phase of the composting period.
FAU - Pedro, M S
AU  - Pedro MS
AD  - Department of Biotechnology, Graduate School of Agricultural and Life Sciences, 
      The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Haruta, S
AU  - Haruta S
FAU - Hazaka, M
AU  - Hazaka M
FAU - Shimada, R
AU  - Shimada R
FAU - Yoshida, C
AU  - Yoshida C
FAU - Hiura, K
AU  - Hiura K
FAU - Ishii, M
AU  - Ishii M
FAU - Igarashi, Y
AU  - Igarashi Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
EDAT- 2005/10/20 09:00
MHDA- 2005/10/20 09:01
CRDT- 2005/10/20 09:00
PHST- 2000/05/08 00:00 [received]
PHST- 2000/11/06 00:00 [accepted]
PHST- 2000/05/08 00:00 [received]
PHST- 2000/11/06 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2005/10/20 09:01 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S1389-1723(01)80059-1 [pii]
AID - 10.1263/jbb.91.159 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2001;91(2):159-65. doi: 10.1263/jbb.91.159.