PMID- 16233349
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20051103
LR  - 20051019
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 94
IP  - 6
DP  - 2002
TI  - In situ identification of microorganisms in biofilm communities.
PG  - 552-6
AB  - Diverse microorganisms form complex microbial communities and usually exist in 
      biofilm communities in both natural environments and engineered systems such as a 
      wastewater treatment process. However, the conventional approach to investigate 
      microbial ecology has not contributed to the understanding and clarification of 
      the structure and function of biofilm communities. Some effective methods have 
      been developed to investigate phylogenetic affiliations, metabolic activities and 
      genetic activities in biofilm communities at the single-cell level. These 
      techniques have been contributing to a better understanding of the spatial 
      organization of biofilm communities and activities in engineered systems. 
      However, further effort is needed to set out the general rules governing 
      community development in biofilm communities and to advance the process 
      performance of engineered systems. This review describes advances and limitations 
      in methodology, particularly focusing on fluorescence in situ hybridization 
      (FISH) and related techniques and the application of these methods to nitrifying 
      biofilms in wastewater treatment processes.
FAU - Aoi, Yoshiteru
AU  - Aoi Y
AD  - Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, 
      Tokyo 169-8555, Japan. yoshiteruaoi@toki.waseda.jp
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
EDAT- 2005/10/20 09:00
MHDA- 2005/10/20 09:01
CRDT- 2005/10/20 09:00
PHST- 2002/07/31 00:00 [received]
PHST- 2002/09/04 00:00 [accepted]
PHST- 2002/07/31 00:00 [received]
PHST- 2002/09/04 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2005/10/20 09:01 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S1389-1723(02)80194-3 [pii]
PST - ppublish
SO  - J Biosci Bioeng. 2002;94(6):552-6.