PMID- 16233390 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20051108 LR - 20191026 IS - 1389-1723 (Print) IS - 1347-4421 (Linking) VI - 95 IP - 2 DP - 2003 TI - Factual analysis of granule flotation in brewery wastewater treatment plants by the fluorescence in situ hybridization method. PG - 188-91 AB - The factors that change the microbial distribution and consequently the flotation of brewery granules were investigated using laboratory-scale upflow anaerobic sludge blanket (UASB) reactors and the fluorescence in situ hybridization (FISH) method. The startup operations of laboratory-scale UASB reactors fed with acetate-based synthetic wastewater, in which the loading rate was maintained at 0.1 gCOD/gVSS/d (Run 1) and increased in a stepwise manner from 0.1 gCOD/gVSS/d to 1.0 gCOD/gVSS/d (Run 2), generated methanogen colonies near the granule surface, while the overloading operation at 1.0 gCOD/gVSS/d from the startup (Run 3) resulted in the formation of methanogen colonies deep in the granules. In each run, a proportion of the granules floated when overloaded at 2.0 gCOD/gVSS/d and circulation was stopped. The ratio of floating granules increased as the methanogen-growing region increased. On the other hand, the Bacteria layer on the granule surface, which is also considered as a possible cause of granule flotation, was not formed by the inflow of other organic acids such as propionate and lactate. Glucose caused formation of a 5-microm-thick surface Bacteria layer, but the granules were still resistant to flotation. Interfusing of air under glucose feeding caused the formation of a Bacteria layer over 50 microm thick leading to granule flotation. FAU - Saiki, Yuko AU - Saiki Y AD - Fundamental Research Laboratory, Asahi Breweries, Ltd., 1-1-21 Midori, Moriya 302-0106, Japan. yuko.saiki@asahibeer.co.jp FAU - Katami, Akiko AU - Katami A FAU - Kitagawa, Yasushi AU - Kitagawa Y LA - eng PT - Journal Article PL - Japan TA - J Biosci Bioeng JT - Journal of bioscience and bioengineering JID - 100888800 EDAT- 2005/10/20 09:00 MHDA- 2005/10/20 09:01 CRDT- 2005/10/20 09:00 PHST- 2002/08/05 00:00 [received] PHST- 2002/10/03 00:00 [accepted] PHST- 2002/08/05 00:00 [received] PHST- 2002/10/03 00:00 [accepted] PHST- 2005/10/20 09:00 [pubmed] PHST- 2005/10/20 09:01 [medline] PHST- 2005/10/20 09:00 [entrez] AID - S1389-1723(03)80127-5 [pii] AID - 10.1016/s1389-1723(03)80127-5 [doi] PST - ppublish SO - J Biosci Bioeng. 2003;95(2):188-91. doi: 10.1016/s1389-1723(03)80127-5.