PMID- 16233421
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20051108
LR  - 20191026
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 95
IP  - 4
DP  - 2003
TI  - Isolation and characterization of predominant microorganisms during decomposition 
      of waste materials in a field-scale composter.
PG  - 368-73
AB  - A self-heating field-scale composter treating agro-industrial wastes within a 
      period of 30 d was analyzed by denaturing gradient gel electrophoresis (DGGE) 
      (Pedro et al., J. Biosci. Bioeng., 91, 159-165, 2001). Three major bands were 
      derived from Propionibacterium acnes, Methylobacterium mesophilicum or M. 
      radiotolerans, and Bacillus thermocloacae. Strains MSP09A and MSP06G with close 
      affiliation to P. acnes and B. thermocloacae, respectively, were successfully 
      isolated. Based on quantitative-PCR results, the relative population of MSP09A 
      increased towards the end of the composting process (mesophilic stage) while 
      MSP06G seemed to predominate during the middle period (thermophilic stage). These 
      results correlated highly with their growth temperatures. MSP09A and MSP06G had 
      different metabolic profiles which were largely affected by culture conditions. 
      MSP09A was able to utilize large complex molecules of lipids and proteins. An 
      interspecies relationship in terms of metabolites such as propionic acid was 
      expected between the two microorganisms.
FAU - Pedro, Mannix S
AU  - Pedro MS
AD  - Department of Biotechnology, Graduate School of Agricultural and Life Sciences, 
      The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Haruta, Shin
AU  - Haruta S
FAU - Nakamura, Kohei
AU  - Nakamura K
FAU - Hazaka, Masaru
AU  - Hazaka M
FAU - Ishii, Masaharu
AU  - Ishii M
FAU - Igarashi, Yasuo
AU  - Igarashi Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
EDAT- 2005/10/20 09:00
MHDA- 2005/10/20 09:01
CRDT- 2005/10/20 09:00
PHST- 2002/07/24 00:00 [received]
PHST- 2002/12/14 00:00 [accepted]
PHST- 2002/07/24 00:00 [received]
PHST- 2002/12/14 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2005/10/20 09:01 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S1389-1723(03)80069-5 [pii]
AID - 10.1016/s1389-1723(03)80069-5 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2003;95(4):368-73. doi: 10.1016/s1389-1723(03)80069-5.