PMID- 16233572
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20051107
LR  - 20051019
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 96
IP  - 6
DP  - 2003
TI  - Effect of dilution rate on structure of a mesophilic acetate-degrading 
      methanogenic community during continuous cultivation.
PG  - 547-58
AB  - The community structures of two mesophilic acetate-degrading methanogenic 
      consortia enriched at dilution rates of 0.025 and 0.6 d(-1) were analyzed by 
      fluorescence in situ hybridization (FISH) and phylogenetic analyses based on 16S 
      rDNA clonal sequences and quantitative real-time polymerase chain reaction (PCR). 
      FISH experiments with archaeal and bacterial domain-specific probes showed that 
      archaeal cells were predominant and only a small number of bacterial cells were 
      detected at both dilution rates. In the domain Archaea, the number of cells 
      closely related to Methanosarcina barkeri was shown to be greater at the high 
      dilution rate using FISH with species-specific probes. Taxonomic analyses based 
      on rDNA clonal sequences obtained at the low and high dilution rates showed that 
      43% of 100 clones and 72% of 92 clones, respectively, were affiliated with the 
      domain Archaea and the remainders at each dilution rate were affiliated with the 
      domain Bacteria. Within the domain Archaea, all rDNA clones at both dilution 
      rates were affiliated with the genera Methanosaeta or Methanosarcina of the 
      aceticlastic methanogens. Within the domain Bacteria, the rDNA clones obtained at 
      the low dilution rate were affiliated with four phyla, Firmicutes (36%), 
      Bacteroidetes (9%), Chloroflexi (6%) and candidate division OP12 (5%). The rDNA 
      clones obtained at the high dilution rate were affiliated with four phyla, 
      Firmicutes (16%), Bacteroidetes (8%), Proteobacteria (1%) and candidate division 
      OP12 (3%). Real-time quantitative PCR experiments showed that the number of rDNA 
      sequences affiliated with the genus Methanosarcina was greater at the high 
      dilution rate. In addition, a significant number of rDNA sequences affiliated 
      with the genus Methanoculleus were detected only at the low dilution rate. 
      Detection of a hydrogenotrophic methanogen at the low dilution rate suggests that 
      the syntrophic acetate oxidation by hydrogenotrophic methanogens and 
      acetate-oxidizing bacteria could occur at the low dilution rate.
FAU - Shigematsu, Toru
AU  - Shigematsu T
AD  - Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, 
      Kumamoto City, Kumamoto 860-8555, Japan. shige@kumamoto-u.ac.jp
FAU - Tang, Yueqin
AU  - Tang Y
FAU - Kawaguchi, Hiromi
AU  - Kawaguchi H
FAU - Ninomiya, Kana
AU  - Ninomiya K
FAU - Kijima, Junji
AU  - Kijima J
FAU - Kobayashi, Tsutomu
AU  - Kobayashi T
FAU - Morimura, Shigeru
AU  - Morimura S
FAU - Kida, Kenji
AU  - Kida K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
EDAT- 2005/10/20 09:00
MHDA- 2005/10/20 09:01
CRDT- 2005/10/20 09:00
PHST- 2003/02/03 00:00 [received]
PHST- 2003/09/29 00:00 [accepted]
PHST- 2003/02/03 00:00 [received]
PHST- 2003/09/29 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2005/10/20 09:01 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S1389-1723(04)70148-6 [pii]
AID - 10.1016/S1389-1723(04)70148-6 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2003;96(6):547-58. doi: 10.1016/S1389-1723(04)70148-6.