PMID- 16233832 OWN - NLM STAT- MEDLINE DCOM- 20051219 LR - 20081121 IS - 1389-1723 (Print) IS - 1347-4421 (Linking) VI - 99 IP - 6 DP - 2005 Jun TI - Cloning and expression analysis of two catalase genes from Aspergillus oryzae. PG - 562-8 AB - Fungi contain distinct genes encoding the same class of enzyme that are differentially regulated according to conditions. We cloned two catalase genes, catA and catB, from Aspergillus oryzae. The catA gene predicts a 747-amino-acid polypeptide sharing 81% identity with Aspergillus fumigatus catalase (catA) and 77% with Aspergillus nidulans catalase (catA). The catB gene predicts a 725-amino-acid polypeptide sharing 82% identity with A. fumigatus catalase (catB) and 75% with A. nidulans catalase (catB). However, the catA and catB genes share little homology (41%) with one another, suggesting that each gene belongs to a distinct gene family. Overexpression studies demonstrated that both genes encode a functional catalase. Promoter assays indicated that the catA gene is developmentally regulated as it was preferentially expressed in solid-state cultures undergoing sporulation. However, its expression was not affected by hydrogen peroxide treatment. Conversely, the catB gene was highly expressed under all culture conditions tested, and it was induced by hydrogen peroxide treatment. These results suggest that the catB gene may be mainly used for detoxification of oxidative stress while the catA gene may have another role such as chaperoning proteins in the spore. FAU - Hisada, Hiromoto AU - Hisada H AD - Research Institute, Gekkeikan Sake Co. Ltd., 24 Shimotoba-koyanagi-cho, Fushimi-ku, Kyoto 612-8361, Japan. hisada@gekkeikan.co.jp FAU - Hata, Yoji AU - Hata Y FAU - Kawato, Akitsugu AU - Kawato A FAU - Abe, Yasuhisa AU - Abe Y FAU - Akita, Osamu AU - Akita O LA - eng PT - Journal Article PL - Japan TA - J Biosci Bioeng JT - Journal of bioscience and bioengineering JID - 100888800 RN - 0 (Recombinant Proteins) RN - EC 1.11.1.6 (Catalase) SB - IM MH - Amino Acid Sequence MH - Aspergillus oryzae/*enzymology/genetics MH - Catalase/*chemistry/genetics/*metabolism MH - Cloning, Molecular MH - Escherichia coli/genetics/*metabolism MH - Gene Expression Regulation, Enzymologic/physiology MH - Gene Expression Regulation, Fungal/physiology MH - Molecular Sequence Data MH - Oxidative Stress/*physiology MH - Promoter Regions, Genetic/genetics MH - Recombinant Proteins/chemistry/metabolism MH - Sequence Homology, Amino Acid EDAT- 2005/10/20 09:00 MHDA- 2005/12/20 09:00 CRDT- 2005/10/20 09:00 PHST- 2005/01/27 00:00 [received] PHST- 2005/03/10 00:00 [accepted] PHST- 2005/10/20 09:00 [pubmed] PHST- 2005/12/20 09:00 [medline] PHST- 2005/10/20 09:00 [entrez] AID - S1389-1723(05)70409-6 [pii] AID - 10.1263/jbb.99.562 [doi] PST - ppublish SO - J Biosci Bioeng. 2005 Jun;99(6):562-8. doi: 10.1263/jbb.99.562.