PMID- 16233841
OWN - NLM
STAT- MEDLINE
DCOM- 20051219
LR  - 20061115
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 99
IP  - 6
DP  - 2005 Jun
TI  - D-Amino acid dipeptide production utilizing D-alanine-D-alanine ligases with 
      novel substrate specificity.
PG  - 623-8
AB  - D-Alanine-D-alanine ligase (Ddl) is an important enzyme in the synthesis of 
      bacterial peptidoglycan. The genes encoding Ddls from Escherichia coli K12 
      (EcDdlB), Oceanobacillus iheyensis JCM 11309 (OiDdl), Synechocystis sp. PCC 6803 
      (SsDdl) and Thermotoga maritima ATCC 43589 (TmDdl), the genomic DNA sequences of 
      which have been determined, were cloned and the substrate specificities of these 
      recombinant Ddls were investigated. Although OiDdl had a high substrate 
      specificity for D-alanine; EcDdlB, SsDdl and TmDdl showed broad substrate 
      specificities for D-serine, D-threonine, D-cysteine and glycine, in addition to 
      D-alanine. Four D-amino acid dipeptides were produced using EcDdlB, and D-amino 
      acid homo-dipeptides were successfully produced at high yields except for 
      D-threonyl-D-threonine.
FAU - Sato, Masaru
AU  - Sato M
AD  - Department of Applied Chemistry, School of Science and Engineering, Waseda 
      University, 3-4-1 Ohkubo, Shinjuku-ku 169-8555, Tokyo, Japan.
FAU - Kirimura, Kohtaro
AU  - Kirimura K
FAU - Kino, Kuniki
AU  - Kino K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (Dipeptides)
RN  - 0 (Recombinant Proteins)
RN  - EC 6.3.2.- (Peptide Synthases)
RN  - EC 6.3.2.4 (D-alanylalanine synthetase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cloning, Molecular
MH  - Dipeptides/*chemistry/*metabolism
MH  - Enzyme Activation
MH  - Escherichia coli/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Peptide Synthases/*chemistry/genetics/*metabolism
MH  - Protein Engineering/*methods
MH  - Recombinant Proteins/metabolism
MH  - Stereoisomerism
MH  - Substrate Specificity
EDAT- 2005/10/20 09:00
MHDA- 2005/12/20 09:00
CRDT- 2005/10/20 09:00
PHST- 2005/01/24 00:00 [received]
PHST- 2005/04/01 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2005/12/20 09:00 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S1389-1723(05)70418-7 [pii]
AID - 10.1263/jbb.99.623 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2005 Jun;99(6):623-8. doi: 10.1263/jbb.99.623.