PMID- 16233860
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20051117
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 100
IP  - 1
DP  - 2005 Jul
TI  - Heat immunotherapy using magnetic nanoparticles and dendritic cells for 
      T-lymphoma.
PG  - 112-5
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that play important 
      roles in regulating immune responses in cancer. Immunotherapy using these 
      immunocytes has become an accepted therapeutic modality. We previously reported 
      that hyperthermia using magnetic nanoparticles induces antitumor immunity, which 
      could be activated by adjuvant including cytokines. In the present study, we 
      investigated the therapeutic effects of hyperthermia combined with DC 
      immunotherapy on mouse EL4 T-lymphoma. Magnetite cationic liposomes (MCLs) have a 
      positive surface charge and generate heat in an alternating magnetic field (AMF) 
      due to hysteresis loss. MCLs were injected into an EL4 nodule in C57BL/6 mice, 
      which were subjected to AMF for 30 min. The temperature at the surface of the 
      tumor reached 45 degrees C and was maintained by controlling the magnetic field 
      intensity. Hyperthermia treatment was repeated twice with 24 h intervals. After 
      hyperthermia, immature DCs were directly injected into the EL4 nodule. As a 
      result, complete regression of tumors in 75% (6/8) of the mice was observed, 
      while the percentage of complete regression of tumors was 12.5% (1/8) in the case 
      of mice treated by hyperthermia alone. This novel cancer therapy, which we have 
      termed "heat immunotherapy", may be applicable to patients with advanced 
      malignancies.
FAU - Tanaka, Kouji
AU  - Tanaka K
AD  - Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya 464-8603, Japan.
FAU - Ito, Akira
AU  - Ito A
FAU - Kobayashi, Takeshi
AU  - Kobayashi T
FAU - Kawamura, Tatsuyoshi
AU  - Kawamura T
FAU - Shimada, Shinji
AU  - Shimada S
FAU - Matsumoto, Kazuhiko
AU  - Matsumoto K
FAU - Saida, Toshiaki
AU  - Saida T
FAU - Honda, Hiroyuki
AU  - Honda H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Combined Modality Therapy
MH  - Dendritic Cells/*transplantation
MH  - Hyperthermia, Induced/*methods
MH  - Immunotherapy/*methods
MH  - Lymphoma/*pathology/*therapy
MH  - Magnetics/*therapeutic use
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Nanotubes
MH  - Treatment Outcome
EDAT- 2005/10/20 09:00
MHDA- 2005/11/16 09:00
CRDT- 2005/10/20 09:00
PHST- 2005/01/21 00:00 [received]
PHST- 2005/03/01 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S1389-1723(05)70437-0 [pii]
AID - 10.1263/jbb.100.112 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2005 Jul;100(1):112-5. doi: 10.1263/jbb.100.112.