PMID- 16233867
OWN - NLM
STAT- MEDLINE
DCOM- 20060214
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1093
IP  - 1-2
DP  - 2005 Nov 4
TI  - Determination of mercury species in fish reference materials by gas 
      chromatography-atomic fluorescence detection after closed-vessel 
      microwave-assisted extraction.
PG  - 21-8
AB  - A simple and rapid method has been developed for speciation analysis of inorganic 
      mercury and monomethylmercury (MMHg) in biological tissues. The procedure is 
      based on the quantitative closed-vessel microwave-assisted leaching of mercury 
      from biological samples with an alkaline extractant. The extracted mercury 
      species are ethylated and analysed by capillary gas chromatography coupled to an 
      atomic fluorescence detector via pyrolysis (CGC-pyro-AFS). The coupling between 
      capillary gas chromatography and atomic fluorescence detector was optimized with 
      the aim of minimizing the detection limits and time necessary for the 
      species-selective determination of mercury compounds. The use of closed-vessel 
      microwave-assisted extraction along with no clean-up steps before the ethylation 
      leads to a significant total analysis time decrease with respect to similar 
      procedures. The detection limit was 2 pg for MMHg (as Hg) and 1 pg for inorganic 
      mercury. The method was validated by the analysis of DORM-2 (dogfish muscle) and 
      DOLT-3 (dogfish liver) certified reference materials. The inorganic mercury and 
      methylmercury concentrations found were in good agreement with the certified 
      values. Recovery studies of fish muscle tissue spiked with inorganic mercury and 
      MMHg were done to check the reliability of the method. In all cases satisfactory 
      recoveries (92-105%) were obtained.
FAU - Nevado, Juan Jose Berzas
AU  - Nevado JJ
AD  - Regional Institute for Applied Scientific Research, University of Castilla-La 
      Mancha, E-16071 Ciudad Real, Spain.
FAU - Martin-Doimeadios, Rosa Carmen Rodriguez
AU  - Martin-Doimeadios RC
FAU - Bernardo, Francisco Javier Guzman
AU  - Bernardo FJ
FAU - Moreno, Maria Jimenez
AU  - Moreno MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050826
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - FXS1BY2PGL (Mercury)
SB  - IM
MH  - Animals
MH  - Chromatography, Gas/*methods
MH  - *Dogfish
MH  - Mercury/*analysis/classification
MH  - Microwaves
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2005/10/20 09:00
MHDA- 2006/02/16 09:00
CRDT- 2005/10/20 09:00
PHST- 2005/05/23 00:00 [received]
PHST- 2005/07/12 00:00 [revised]
PHST- 2005/07/18 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2006/02/16 09:00 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S0021-9673(05)01554-2 [pii]
AID - 10.1016/j.chroma.2005.07.054 [doi]
PST - ppublish
SO  - J Chromatogr A. 2005 Nov 4;1093(1-2):21-8. doi: 10.1016/j.chroma.2005.07.054. 
      Epub 2005 Aug 26.