PMID- 16233881
OWN - NLM
STAT- MEDLINE
DCOM- 20060214
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1093
IP  - 1-2
DP  - 2005 Nov 4
TI  - Natural sample fractionation by FlFFF-MALLS-TEM: sample stabilization, 
      preparation, pre-concentration and fractionation.
PG  - 156-66
AB  - Two flow field flow fractionation (FlFFF) systems: symmetrical (SFlFFF) and 
      asymmetrical (ASFlFFF) were evaluated to fractionate river colloids. Samples 
      stability during storage and colloids concentration are the main challenges 
      limiting their fractionation and characterization by FlFFF. A pre-fractionation 
      (<0.45 microm) and addition of a bactericide such as NaN3 into river colloidal 
      samples allowed obtaining stable samples without inducing any modification to 
      their size. Stirred cell ultra-filtration allowed colloidal concentration 
      enrichment of 25-folds. Scanning electron microscope (SEM) micrographs confirmed 
      the gentle pre-concentration of river samples using the ultra-filtration stirred 
      cell. Additionally, larger sample injection volume in the case of SFlFFF and on 
      channel concentration in the case of ASFlFFF were applied to minimize the 
      required pre-concentration. Multi angle laser light scattering (MALLS), and 
      transmission electron microscope (TEM) techniques are used to evaluate FlFFF 
      fractionation behavior and the possible artifacts during fractionation process. 
      This study demonstrates that, FlFFF-MALLS-TEM coupling is a valuable method to 
      fractionate and characterize colloids. Results prove an ideal fractionation 
      behavior in case of Brugeilles sample and steric effect influencing the elution 
      mode in case of Cezerat and Chatillon. Furthermore, comparison of SFlFFF and 
      ASFlFFF fractograms for the same sample shows small differences in particle size 
      distributions.
FAU - Baalousha, M
AU  - Baalousha M
AD  - Center of Development of Applied Geology, University of Bordeaux 1, Bat B18, 
      Avenue des Facultes, 33400 Talence, France. mbalousha@yahoo.co.uk
FAU - Kammer, F V D
AU  - Kammer FV
FAU - Motelica-Heino, M
AU  - Motelica-Heino M
FAU - Le Coustumer, P
AU  - Le Coustumer P
LA  - eng
PT  - Journal Article
DEP - 20050816
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Colloids)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Colloids
MH  - Fractionation, Field Flow/*methods
MH  - Microscopy, Electron, Transmission/*methods
MH  - Spectrophotometry, Ultraviolet/methods
MH  - Water
EDAT- 2005/10/20 09:00
MHDA- 2006/02/16 09:00
CRDT- 2005/10/20 09:00
PHST- 2005/06/01 00:00 [received]
PHST- 2005/07/21 00:00 [revised]
PHST- 2005/07/25 00:00 [accepted]
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2006/02/16 09:00 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S0021-9673(05)01599-2 [pii]
AID - 10.1016/j.chroma.2005.07.103 [doi]
PST - ppublish
SO  - J Chromatogr A. 2005 Nov 4;1093(1-2):156-66. doi: 10.1016/j.chroma.2005.07.103. 
      Epub 2005 Aug 16.