PMID- 16234354
OWN - NLM
STAT- MEDLINE
DCOM- 20060404
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 107
IP  - 4
DP  - 2006 Feb 15
TI  - Phagocytosis of codeveloping megakaryocytic progenitors by dendritic cells in 
      culture with thrombopoietin and tumor necrosis factor-alpha and its possible role 
      in hemophagocytic syndrome.
PG  - 1366-74
AB  - Tumor necrosis factor-alpha (TNF-alpha) and thrombopoietin (TPO) have been shown 
      to induce the differentiation and proliferation of CD34+ cells toward dendritic 
      cells (DCs) in the presence of multiacting cytokines. We hypothesized that the 
      costimulation of TPO and TNF-alpha generates megakaryocytic progenitors and DCs 
      together from human CD34+ cells and that the interaction of these cells may 
      indicate a physiologic and/or a pathologic role of DCs in megakaryopoiesis. When 
      highly purified human CD34+ cells were cultured for 7 days with TPO alone, the 
      generated cells expressed megakaryocytic markers, such as CD41, CD42b, and CD61. 
      The addition of TNF-alpha with TPO remarkably decreased the number of 
      megakaryocytic progenitor cells without affecting the cell yield. Almost half of 
      the cells thus generated expressed CD11c, and most of them were positive for CD4 
      and CD123. Furthermore, CD11c+ cells were found to capture damaged CD61+ cells 
      and to induce autologous T-cell proliferation, although the cytokine productions 
      were low. We also confirmed an engulfment of CD61+ cells and their fragment by 
      CD11c+ cells in bone marrow cells from patients with hemophagocytic syndrome. 
      These findings suggest that DCs generated under megakaryocytic and inflammatory 
      stimuli are involved in megakaryopoiesis and the subsequent immune responses to 
      self-antigens.
FAU - Saito, Kunie
AU  - Saito K
AD  - Department of Internal Medicine III, Akita University School of Medicine, Hondo 
      1-1-1, Akita 010-8543, Japan.
FAU - Hirokawa, Makoto
AU  - Hirokawa M
FAU - Inaba, Kayo
AU  - Inaba K
FAU - Fukaya, Hiroshi
AU  - Fukaya H
FAU - Kawabata, Yoshinari
AU  - Kawabata Y
FAU - Komatsuda, Atsushi
AU  - Komatsuda A
FAU - Yamashita, Junsuke
AU  - Yamashita J
FAU - Sawada, Kenichi
AU  - Sawada K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051018
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9014-42-0 (Thrombopoietin)
SB  - IM
MH  - Aged
MH  - Antigens, CD/blood
MH  - Antigens, CD34/blood
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*physiology
MH  - Female
MH  - Humans
MH  - Lymphohistiocytosis, Hemophagocytic/blood/immunology/*physiopathology
MH  - Male
MH  - Megakaryocytes/*cytology/drug effects/*physiology
MH  - Middle Aged
MH  - Phagocytosis
MH  - Thrombopoietin/*pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2005/10/20 09:00
MHDA- 2006/04/06 09:00
CRDT- 2005/10/20 09:00
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2006/04/06 09:00 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - S0006-4971(20)66386-8 [pii]
AID - 10.1182/blood-2005-08-3155 [doi]
PST - ppublish
SO  - Blood. 2006 Feb 15;107(4):1366-74. doi: 10.1182/blood-2005-08-3155. Epub 2005 Oct 
      18.