PMID- 16234988
OWN - NLM
STAT- MEDLINE
DCOM- 20060126
LR  - 20181113
IS  - 0167-594X (Print)
IS  - 0167-594X (Linking)
VI  - 75
IP  - 2
DP  - 2005 Nov
TI  - Dendritic cells pulsed with total tumor RNA for activation NK-like T cells 
      against glioblastoma multiforme.
PG  - 111-8
AB  - Dendritic cells (DCs) are potent antigen presenting cells and play critical role 
      in T cell-mediated immunity. DCs have been shown to induce strong anti-tumor 
      responses both in vitro and in vivo. Their efficacies in tumor therapy are being 
      investigated in clinical trials. Previous evidence has shown that these DCs 
      enhance the cytotoxicity of NK cells. We generated NK-like T cells 
      (CD3(+)CD56(+)), a novel type of effector cells differentiated from normal 
      lymphocyte, which is now being used for adoptive immunotherapy in clinical 
      trials. This study aimed to elucidate the effects of NK-like T cells after 
      co-culturing with DCs against tumor cells. The result revealed that tumor-derived 
      RNA-pulsed DCs can enhance the immune responses of NK-like T cells against 
      glioblastoma multiforme cell line but these effector cells did not appear to have 
      the cytotoxic effect against normal cells (human umbilical vein endothelial cells 
      (HUVEC) and fibroblasts) in vitro. This study may be beneficial for the 
      development of new immunologic effector cells for using in adoptive immunotherapy 
      for glioblastoma multiforme in the future.
FAU - Vichchatorn, Prachya
AU  - Vichchatorn P
AD  - Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 
      Thailand.
FAU - Wongkajornsilp, Adisak
AU  - Wongkajornsilp A
FAU - Petvises, Sawang
AU  - Petvises S
FAU - Tangpradabkul, Sumalee
AU  - Tangpradabkul S
FAU - Pakakasama, Samart
AU  - Pakakasama S
FAU - Hongeng, Suradej
AU  - Hongeng S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurooncol
JT  - Journal of neuro-oncology
JID - 8309335
RN  - 0 (CD3 Complex)
RN  - 0 (CD56 Antigen)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Antigen Presentation
MH  - CD3 Complex/immunology
MH  - CD56 Antigen/immunology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Cytotoxicity Tests, Immunologic/methods
MH  - Dendritic Cells/*immunology
MH  - Endothelial Cells/cytology
MH  - Endothelium, Vascular/cytology
MH  - Glioblastoma/*therapy
MH  - Humans
MH  - Immunophenotyping
MH  - Immunotherapy, Adoptive/methods
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - *RNA, Neoplasm
MH  - T-Lymphocytes/*immunology
MH  - Umbilical Veins/cytology
EDAT- 2005/10/20 09:00
MHDA- 2006/01/27 09:00
CRDT- 2005/10/20 09:00
PHST- 2005/10/20 09:00 [pubmed]
PHST- 2006/01/27 09:00 [medline]
PHST- 2005/10/20 09:00 [entrez]
AID - 10.1007/s11060-005-2317-2 [doi]
PST - ppublish
SO  - J Neurooncol. 2005 Nov;75(2):111-8. doi: 10.1007/s11060-005-2317-2.