PMID- 16235535
OWN - NLM
STAT- MEDLINE
DCOM- 20060919
LR  - 20081121
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 36
IP  - 5
DP  - 2005 Sep
TI  - [Expression and function of interleukin-18 in mouse dextran sulfate sodium 
      colitis].
PG  - 676-8
AB  - OBJECTIVE: Interleukin-18 (IL-18) is a proinflammatory cytokine that induces Th1 
      cytokines production and may play a role in the pathogenesis of inflammatory 
      bowel diseases. This study was aimed at detecting the expression and role of 
      IL-18 in colonic mucosa of dextran sulfate sodium (DSS) colitic mice. METHODS: 
      Expression of IL-18 in colon of DSS colitis was determined by 
      immunohistochemistry. Extract and supernatant of intestinal epithelial cells 
      (IEC) from colon of DSS colitis mice were used to stimulate splenic mononuclear 
      cells (SMC); IFN-gamma and IL-2 concentrations in supernatant of SMC were 
      measured by ELISA. The levels of expression of IL-18 in extract or supernatant of 
      IEC were examined by Western blot. RESULTS: Colon epithelial cells and part of 
      lamina propria mononuclear cells were stained positive for IL-18 antibody. 
      Stimulation assay and Western blot indicated that IL-18 positive proteins were 
      present in both IEC extract and supernatant. CONCLUSION: Colon epithelial cells 
      in mice with DSS colitis express and secret pro IL-18 and active IL-18.
FAU - Wen, Zhong-Hui
AU  - Wen ZH
AD  - Department of Gastroenterology, West China Hospital of Sichuan University, 
      Chengdu 610041, China.
FAU - Quyang, Qin
AU  - Quyang Q
FAU - Zhang, Hu
AU  - Zhang H
FAU - Yi, Zhi-Hui
AU  - Yi ZH
FAU - Wang, Yu-Fang
AU  - Wang YF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-2)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Colitis/chemically induced/*metabolism
MH  - Colon/metabolism
MH  - Dextran Sulfate
MH  - Interferon-gamma/metabolism
MH  - Interleukin-18/*biosynthesis/physiology
MH  - Interleukin-2/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2005/10/21 09:00
MHDA- 2006/09/20 09:00
CRDT- 2005/10/21 09:00
PHST- 2005/10/21 09:00 [pubmed]
PHST- 2006/09/20 09:00 [medline]
PHST- 2005/10/21 09:00 [entrez]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Sep;36(5):676-8.