PMID- 16240240
OWN - NLM
STAT- MEDLINE
DCOM- 20060316
LR  - 20230706
IS  - 1525-3961 (Print)
IS  - 1438-7573 (Electronic)
IS  - 1438-7573 (Linking)
VI  - 6
IP  - 4
DP  - 2005 Dec
TI  - Immortalized mouse inner ear cell lines demonstrate a role for chemokines in 
      promoting the growth of developing statoacoustic ganglion neurons.
PG  - 355-67
AB  - The target-derived factors necessary for promoting initial outgrowth from the 
      statoacoustic ganglion (SAG) to the inner ear have not been fully characterized. 
      In the present study, conditioned medium from embryonic Immortomouse inner ear 
      cell lines that maintain many characteristics of developing inner ear sensory 
      epithelia were screened for neurite-promoting activity. Conditioned medium found 
      to be positive for promoting SAG neurite outgrowth and neuronal survival was then 
      tested for the presence of chemokines, molecules that have not previously been 
      investigated for promoting SAG outgrowth. One candidate molecule, monocyte 
      chemotactic protein 1 (MCP-1), was detected in the conditioned medium and 
      subsequently localized to mouse hair cells by immunocytochemistry. In vitro 
      studies demonstrated that function-blocking MCP-1 antibodies decreased the amount 
      of SAG neurite outgrowth induced by the conditioned medium and that subsequent 
      addition of MCP-1 protein was able to promote outgrowth when added to the 
      antibody-treated conditioned medium. The use of the Immortomouse cell lines 
      proved valuable in identifying this candidate cofactor that promotes outgrowth of 
      early-stage SAG nerve fibers and is expressed in embryonic hair cells.
FAU - Bianchi, Lynne M
AU  - Bianchi LM
AD  - Neuroscience Department, Oberlin College, Oberlin, OH 44074, USA. 
      lynne.bianchi@oberlin.edu
FAU - Daruwalla, Zeeba
AU  - Daruwalla Z
FAU - Roth, Therese M
AU  - Roth TM
FAU - Attia, Naweah P
AU  - Attia NP
FAU - Lukacs, Nicholas W
AU  - Lukacs NW
FAU - Richards, Ayo-Lynn
AU  - Richards AL
FAU - White, Ian O
AU  - White IO
FAU - Allen, Susan J
AU  - Allen SJ
FAU - Barald, Kate F
AU  - Barald KF
LA  - eng
GR  - R01 DC004184/DC/NIDCD NIH HHS/United States
GR  - R15 DC005587/DC/NIDCD NIH HHS/United States
GR  - R01DC04184/DC/NIDCD NIH HHS/United States
GR  - R15DC05587/DC/NIDCD NIH HHS/United States
GR  - R01DC05939/DC/NIDCD NIH HHS/United States
GR  - R01 DC005939/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Assoc Res Otolaryngol
JT  - Journal of the Association for Research in Otolaryngology : JARO
JID - 100892857
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Proliferation
MH  - Chemokine CCL2/analysis/physiology
MH  - Chemokine CCL5/analysis/physiology
MH  - Chemokines/*physiology
MH  - Chemotaxis, Leukocyte
MH  - Chick Embryo
MH  - Culture Media, Conditioned
MH  - Ear, Inner/*cytology
MH  - Ganglia/*cytology
MH  - Mice
MH  - Monocytes/immunology
MH  - Neurites/physiology
MH  - Tumor Necrosis Factor-alpha/analysis/physiology
PMC - PMC2504622
EDAT- 2005/10/22 09:00
MHDA- 2006/03/17 09:00
PMCR- 2006/12/01
CRDT- 2005/10/22 09:00
PHST- 2004/11/30 00:00 [received]
PHST- 2005/07/20 00:00 [accepted]
PHST- 2005/10/22 09:00 [pubmed]
PHST- 2006/03/17 09:00 [medline]
PHST- 2005/10/22 09:00 [entrez]
PHST- 2006/12/01 00:00 [pmc-release]
AID - 13 [pii]
AID - 10.1007/s10162-005-0013-8 [doi]
PST - ppublish
SO  - J Assoc Res Otolaryngol. 2005 Dec;6(4):355-67. doi: 10.1007/s10162-005-0013-8.