PMID- 16240696
OWN - NLM
STAT- MEDLINE
DCOM- 20051109
LR  - 20220310
IS  - 1121-7138 (Print)
IS  - 1121-7138 (Linking)
VI  - 28
IP  - 3
DP  - 2005 Jul
TI  - The prevalence and molecular typing of enterotoxigenic Escherichia coli strains 
      isolated from diarrheic stools in Malatya, Turkey.
PG  - 237-43
AB  - This study was performed from June 2002 to November 2003 year in Malatya, eastern 
      Turkey. Stools of 172 diarrheic patients and 90 healthy controls were analysed 
      for enterotoxigenic Escherichia coli (ETEC). Heat-labile (LT) and heat-stable 
      (ST) toxins were investigated by passive latex agglutination and enzyme 
      immunoassay, respectively. Nine ETEC strains were isolated from 172 diarrheic 
      stools (5.2%). Seven of the ETEC strains (10.1%) were isolated from 69 children 
      in the 0-5 year age group. Two of these pediatric isolates were ST positive 
      (2.9%) and five were LT positive (7.2%). ETEC was not isolated in the 6-18 year 
      age group. Two ST producing E. coli strains were detected in diarrheic adult 
      patients (> 18 years). In the 90 controls, two ETEC strains were detected (2.2%). 
      One of them was a LT producer (1.1%) and the other was a ST producer (1.1%). E. 
      coli strains producing both toxins simultaneously were not observed. ETEC 
      positivity was higher in the diarrheic group than in the control group but 
      statistically not significant (p > 0.05). The rate of resistance among ETEC 
      strains to cefuroxime axetil, ampicillin, piperacillin, and 
      trimethoprim-sulfamethoxazole was 72.7%, 54.5%, 45.5%, and 36.4%, respectively 
      whereas the resistance rate to the same antibiotics in non-ETEC strains was 14%, 
      62%, 54%, and 66%, respectively. All ETEC isolates were intermediately resistant 
      to cephalothin and fully susceptible to other antibiotics tested. Typing of the 
      ETEC strains was done by arbitrary primed polymerase chain reaction (AP-PCR). 
      Only two LT strains of the 11 typed strains had a unique profile. The remaining 
      nine were mixed LT and ST strains and divided into two groups. The first group 
      had three strains having a similarity coefficient ranging from 70-90%. The other 
      one had six strains, five of them were similar and one was subtype isolate. It 
      can be concluded that ETEC strains might be considerably important 
      enteropathogens especially in pediatric patients in the 0-5 year age group. High 
      clonal relation indicated that ETEC strains were epidemiologically related.
FAU - Ozerol, Ibrahim Halil
AU  - Ozerol IH
AD  - Department of Medical Microbiology, Faculty of Medicine, Inonu University, 
      Malatya, Turkey.
FAU - Bayraktar, Mehmet Refik
AU  - Bayraktar MR
FAU - Iseri, Latife
AU  - Iseri L
FAU - Otlu, Barifl
AU  - Otlu B
FAU - Durmaz, Riza
AU  - Durmaz R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - New Microbiol
JT  - The new microbiologica
JID - 9516291
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Toxins/*analysis
MH  - Child
MH  - Child, Preschool
MH  - Cluster Analysis
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/analysis/isolation & purification
MH  - Diarrhea/epidemiology/*microbiology
MH  - Drug Resistance, Bacterial
MH  - Enterotoxins/*analysis
MH  - Escherichia coli/classification/genetics/isolation & purification/*pathogenicity
MH  - Escherichia coli Infections/epidemiology/*microbiology
MH  - Escherichia coli Proteins/*analysis
MH  - Feces/*microbiology
MH  - Genotype
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Infant
MH  - Infant, Newborn
MH  - Latex Fixation Tests
MH  - Microbial Sensitivity Tests
MH  - Molecular Epidemiology
MH  - Polymerase Chain Reaction
MH  - Turkey/epidemiology
EDAT- 2005/10/26 09:00
MHDA- 2005/11/10 09:00
CRDT- 2005/10/26 09:00
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2005/11/10 09:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
PST - ppublish
SO  - New Microbiol. 2005 Jul;28(3):237-43.