PMID- 16241060
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20161124
IS  - 1000-0593 (Print)
IS  - 1000-0593 (Linking)
VI  - 25
IP  - 7
DP  - 2005 Jul
TI  - [Determination of vitamin B12 concentration by fluorescence quenching with 
      acridine orange-rhodamine 6G energy transfer system].
PG  - 1080-2
AB  - An energy transfer technique between acridine orange (AO) and rhodamine 6G (R6G) 
      was studied, and the optimum experimental conditions of energy transfer were 
      defined. It was found that the effective energy transfer could occur between AO 
      and R6G in the dodecylbenzene sodium sulfonate solution with Na2 HPO4-citric acid 
      buffer solution at pH 5.0. The fluorescence intensity of AO-R6G system was 
      diminished by vitamin B12 in an alkalescence medium. Based on the AO-R6G energy 
      transfer system anovel fluorescence quenching method for the determination of 
      vitamin B12 has been developed. Under optimal conditious, the linear range of 
      calibration curves for the determination of vitamin B12 was 0-3.0 x 10(-5) mol x 
      L(-1). The detection limits were 4.8 x 10(-7) mol x L(-1) for Vitamin B12. Among 
      six times of determination, the relative standard deviation was 0.51%-0.64%, and 
      the recovery was 98.40% -103.62%. The method features good recurrence, rapidity 
      of reaction, good stability, and few interfering substances. It can be 
      satisfactorily used in the determination of the injection content of vitamin B12.
FAU - Liu, Bao-sheng
AU  - Liu BS
AD  - The Center of Physics and Chemistry, Hebei Provincial Key Laboratory of 
      Analytical Science and Technology, Hebei University, Baoding 071002, China.
FAU - Gao, Jing
AU  - Gao J
FAU - Yang, Geng-liang
AU  - Yang GL
LA  - chi
PT  - Journal Article
PL  - China
TA  - Guang Pu Xue Yu Guang Pu Fen Xi
JT  - Guang pu xue yu guang pu fen xi = Guang pu
JID - 9424805
RN  - 0 (Rhodamines)
RN  - 037VRW83CF (rhodamine 6G)
RN  - F30N4O6XVV (Acridine Orange)
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Calibration
MH  - Energy Transfer
MH  - *Fluorescence
MH  - Reproducibility of Results
MH  - Rhodamines/*chemistry
MH  - Spectrometry, Fluorescence
MH  - Vitamin B 12/*analysis/chemistry
EDAT- 2005/10/26 09:00
MHDA- 2010/06/11 06:00
CRDT- 2005/10/26 09:00
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
PST - ppublish
SO  - Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Jul;25(7):1080-2.