PMID- 16242161
OWN - NLM
STAT- MEDLINE
DCOM- 20060522
LR  - 20131121
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 595
IP  - 1-2
DP  - 2006 Mar 20
TI  - SMC1 inhibition results in FRA3B expression but has no effect on its delayed 
      replication.
PG  - 23-8
AB  - Cellular processes involved in fragile site expression have been investigated by 
      studying the effect on the replication pattern of the commonest fragile site 
      FRA3B of RNA interference (RNAi)-mediated sister maintenance chromosome 1 (SMC1) 
      inhibition in normal human fibroblasts. Replication timing of FRA3B in G2 was 
      studied by bromodeoxyuridine (BrdU) labeling for the final 2h of cell culture 
      whereas in the S phase was investigated by a fluorescence in situ hybridization 
      (FISH)-based approach through the analysis of clones spanning the FRA3B region. 
      Results showed that FRA3B is normally late replicated even though it is not 
      expressed in untreated cells. On the other hand, SMC1 inhibition leads to FRA3B 
      expression even if the percent of late replicated cells is comparable to control 
      cells. These results obtained by analysing the commonest fragile site suggest 
      that SMC1 plays a role in protecting late replicating regions from stresses 
      occurring in the final steps of genome replication and that delayed replication 
      is necessary but not sufficient for inducing fragile site expression.
FAU - Focarelli, Maria Luisa
AU  - Focarelli ML
AD  - Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, 
      Dipartimento Genoma Umano, Via Fratelli Cervi, 93, 20090 Segrate, Milan, Italy.
FAU - Montagna, Cristina
AU  - Montagna C
FAU - Colombo, Roberto
AU  - Colombo R
FAU - Ried, Thomas
AU  - Ried T
FAU - Vezzoni, Paolo
AU  - Vezzoni P
FAU - Musio, Antonio
AU  - Musio A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (fragile histidine triad protein)
RN  - 0 (structural maintenance of chromosome protein 1)
RN  - 9007-49-2 (DNA)
RN  - EC 3.6.- (Acid Anhydride Hydrolases)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Acid Anhydride Hydrolases/*genetics
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Cycle Proteins/*antagonists & inhibitors/metabolism
MH  - Cells, Cultured
MH  - Chromosomal Proteins, Non-Histone/*antagonists & inhibitors/metabolism
MH  - Chromosomes, Human, Pair 3/genetics
MH  - DNA/biosynthesis
MH  - DNA Replication Timing/*genetics
MH  - Fibroblasts/cytology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Neoplasm Proteins/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - S Phase/genetics
EDAT- 2005/10/26 09:00
MHDA- 2006/05/23 09:00
CRDT- 2005/10/26 09:00
PHST- 2005/06/02 00:00 [received]
PHST- 2005/08/25 00:00 [revised]
PHST- 2005/09/14 00:00 [accepted]
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2006/05/23 09:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
AID - S0027-5107(05)00402-1 [pii]
AID - 10.1016/j.mrfmmm.2005.09.002 [doi]
PST - ppublish
SO  - Mutat Res. 2006 Mar 20;595(1-2):23-8. doi: 10.1016/j.mrfmmm.2005.09.002.