PMID- 16245329
OWN - NLM
STAT- MEDLINE
DCOM- 20060317
LR  - 20181113
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 235
IP  - 1
DP  - 2006 Jan
TI  - Transforming growth factor-beta stimulates epithelial-mesenchymal transformation 
      in the proepicardium.
PG  - 50-9
AB  - The proepicardium (PE) migrates over the heart and forms the epicardium. A subset 
      of these PE-derived cells undergoes epithelial-mesenchymal transformation (EMT) 
      and gives rise to cardiac fibroblasts and components of the coronary vasculature. 
      We report that transforming growth factor-beta (TGFbeta) 1 and TGFbeta2 increase 
      EMT in PE explants as measured by invasion into a collagen gel, loss of 
      cytokeratin expression, and redistribution of ZO1. The type I TGFbeta receptors 
      ALK2 and ALK5 are both expressed in the PE. However, only constitutively active 
      (ca) ALK2 stimulates PE-derived epithelial cell activation, the first step in 
      transformation, whereas caALK5 stimulates neither activation nor transformation 
      in PE explants. Overexpression of Smad6, an inhibitor of ALK2 signaling, inhibits 
      epithelial cell activation, whereas BMP7, a known ligand for ALK2, has no effect. 
      These data demonstrate that TGFbeta stimulates transformation in the PE and 
      suggest that ALK2 partially mediates this effect.
CI  - 2005 Wiley-Liss, Inc.
FAU - Olivey, Harold E
AU  - Olivey HE
AD  - Department of Pharmacology, Vanderbilt University Medical Center, Nashville, 
      Tennessee 37232-6600, USA.
FAU - Mundell, Nathan A
AU  - Mundell NA
FAU - Austin, Anita F
AU  - Austin AF
FAU - Barnett, Joey V
AU  - Barnett JV
LA  - eng
GR  - T32 GM007628/GM/NIGMS NIH HHS/United States
GR  - HL076133/HL/NHLBI NIH HHS/United States
GR  - HL52922/HL/NHLBI NIH HHS/United States
GR  - HL067105/HL/NHLBI NIH HHS/United States
GR  - T32 HL076133/HL/NHLBI NIH HHS/United States
GR  - P01 HL067105-05/HL/NHLBI NIH HHS/United States
GR  - R01 HL052922-10/HL/NHLBI NIH HHS/United States
GR  - GM007628/GM/NIGMS NIH HHS/United States
GR  - R01 HL052922/HL/NHLBI NIH HHS/United States
GR  - P01 HL067105/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of 
      Anatomists
JID - 9201927
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Smad6 Protein)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Chick Embryo
MH  - Epithelium/*embryology/metabolism
MH  - Fibroblast Growth Factor 1/physiology
MH  - Fibroblast Growth Factor 7/physiology
MH  - Genes, Reporter
MH  - Membrane Proteins/biosynthesis/genetics/metabolism
MH  - Mesoderm/cytology/*metabolism
MH  - Pericardium/cytology/*embryology/metabolism
MH  - Phosphoproteins/biosynthesis/genetics/metabolism
MH  - Receptors, Transforming Growth Factor beta/genetics/metabolism
MH  - Smad6 Protein/biosynthesis/genetics
MH  - Transforming Growth Factor beta/*physiology
MH  - Zonula Occludens-1 Protein
PMC - PMC3160345
MID - NIHMS69727
EDAT- 2005/10/26 09:00
MHDA- 2006/03/18 09:00
PMCR- 2011/08/23
CRDT- 2005/10/26 09:00
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2006/03/18 09:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
PHST- 2011/08/23 00:00 [pmc-release]
AID - 10.1002/dvdy.20593 [doi]
PST - ppublish
SO  - Dev Dyn. 2006 Jan;235(1):50-9. doi: 10.1002/dvdy.20593.