PMID- 1624585
OWN - NLM
STAT- MEDLINE
DCOM- 19920807
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 30
IP  - 6
DP  - 1992 Jun
TI  - Sensitive and specific polymerase chain reaction assays for diagnosis of human 
      T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infections in 
      HTLV-I/II-seropositive individuals.
PG  - 1605-7
AB  - To confirm and differentiate between human T-cell lymphotropic virus type I 
      (HTLV-I) and HTLV-II infections, we analyzed by polymerase chain reaction (PCR) 
      samples of peripheral blood lymphocytes from 98 individuals seropositive for 
      HTLV-I/II using pol (SK110/111) and tax (SK43/44) consensus primer pairs. A total 
      of 96 samples (97.9%) were positive by the tax generic probe, while 95 were typed 
      by the HTLV-I and HTLV-II pol probes. The three pol-negative samples were 
      successfully amplified and typed by nested PCR with primers internal to SK110 and 
      SK111. Results of PCR with a lysate of leukocyte nuclei obtained by whole blood 
      lysis were comparable to those obtained with peripheral blood lymphocytes from 16 
      HTLV-seropositive subjects.
FAU - Heneine, W
AU  - Heneine W
AD  - Retrovirus Disease Branch, Centers for Disease Control, Atlanta, Georgia 30333.
FAU - Khabbaz, R F
AU  - Khabbaz RF
FAU - Lal, R B
AU  - Lal RB
FAU - Kaplan, J E
AU  - Kaplan JE
LA  - eng
SI  - GENBANK/J02029
SI  - GENBANK/M10060
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
GS  - gag
GS  - pol
GS  - tax
MH  - Base Sequence
MH  - DNA, Viral/*genetics
MH  - Genes, gag
MH  - Genes, pX
MH  - Genes, pol
MH  - HTLV-I Infections/*diagnosis
MH  - HTLV-II Infections/*diagnosis
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Human T-lymphotropic virus 2/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
PMC - PMC265343
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
PMCR- 1992/06/01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
PHST- 1992/06/01 00:00 [pmc-release]
AID - 10.1128/jcm.30.6.1605-1607.1992 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1992 Jun;30(6):1605-7. doi: 10.1128/jcm.30.6.1605-1607.1992.