PMID- 16246309
OWN - NLM
STAT- MEDLINE
DCOM- 20060112
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 338
IP  - 2
DP  - 2005 Dec 16
TI  - CPT1alpha over-expression increases long-chain fatty acid oxidation and reduces 
      cell viability with incremental palmitic acid concentration in 293T cells.
PG  - 757-61
AB  - To test the cellular response to an increased fatty acid oxidation, we generated 
      a vector for an inducible expression of the rate-limiting enzyme carnitine 
      palmitoyl-transferase 1alpha (CPT1alpha). Human embryonic 293T kidney cells were 
      transiently transfected and expression of the CPT1alpha transgene in the tet-on 
      vector was activated with doxycycline. Fatty acid oxidation was measured by 
      determining the conversion of supplemented, synthetic cis-10-heptadecenoic acid 
      (C17:1n-7) to C15:ln-7. CPT1alpha over-expression increased mitochondrial 
      long-chain fatty acid oxidation about 6-fold. Addition of palmitic acid (PA) 
      decreased viability of CPT1alpha over-expressing cells in a 
      concentration-dependent manner. Both, PA and CPT1alpha over-expression increased 
      cell death. Interestingly, PA reduced total cell number only in cells 
      over-expressing CPT1alpha, suggesting an effect on cell proliferation that 
      requires PA translocation across the mitochondrial inner membrane. This inducible 
      expression system should be well suited to study the roles of CPT1 and fatty acid 
      oxidation in lipotoxicity and metabolism in vivo.
FAU - Jambor de Sousa, Ulrike L
AU  - Jambor de Sousa UL
AD  - Institute of Animal Sciences, Swiss Federal Institute of Technology, Zurich, 
      Switzerland.
FAU - Koss, Michael D
AU  - Koss MD
FAU - Fillies, Marion
AU  - Fillies M
FAU - Gahl, Anja
AU  - Gahl A
FAU - Scheeder, Martin R L
AU  - Scheeder MR
FAU - Cardoso, M Cristina
AU  - Cardoso MC
FAU - Leonhardt, Heinrich
AU  - Leonhardt H
FAU - Geary, Nori
AU  - Geary N
FAU - Langhans, Wolfgang
AU  - Langhans W
FAU - Leonhardt, Monika
AU  - Leonhardt M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051013
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Fatty Acids)
RN  - 0 (Recombinant Proteins)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
SB  - IM
MH  - Carnitine O-Palmitoyltransferase/genetics/*metabolism
MH  - Cell Line
MH  - Cell Survival/physiology
MH  - Fatty Acids/*metabolism
MH  - Gene Expression Regulation/physiology
MH  - Humans
MH  - Kidney/*cytology/*metabolism
MH  - Lipid Peroxidation/physiology
MH  - Oxidation-Reduction
MH  - Palmitic Acid/*metabolism
MH  - Recombinant Proteins/metabolism
EDAT- 2005/10/26 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/10/26 09:00
PHST- 2005/09/29 00:00 [received]
PHST- 2005/10/03 00:00 [accepted]
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
AID - S0006-291X(05)02236-9 [pii]
AID - 10.1016/j.bbrc.2005.10.016 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2005 Dec 16;338(2):757-61. doi: 
      10.1016/j.bbrc.2005.10.016. Epub 2005 Oct 13.