PMID- 16246374
OWN - NLM
STAT- MEDLINE
DCOM- 20060309
LR  - 20061115
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 78
IP  - 10
DP  - 2006 Feb 2
TI  - Renal ischemia/reperfusion injury inhibits differentiation of dendritic cells 
      derived from bone marrow monocytes in rats.
PG  - 1121-8
AB  - Dendritic cells (DCs) are impacted by surgical injury, exercise, and other 
      physiological stressors. This study aims to determine whether renal I/R injury 
      affects 1) the differentiation of myeloid DCs from bone marrow monocytes (BMMos) 
      and the maturation and activation state of these DCs and 2) DC infiltration of 
      kidney. Sprague-Dawley rats were subjected to I/R injury or sham-operated. 
      Creatinine clearance was monitored daily during the 14 d of reperfusion that 
      followed the ischemic insult. At 2 and 14 d of reperfusion, the following were 
      assessed 1) properties of BMMo-derived DCs (i.e., the amount of generated DCs, 
      differentiation state markers [CD11c, CD80, CD86, and Ia], and functional state 
      [MLR and amount of IL-12 produced]), and 2) the presence of DCs in the kidney. 
      Numbers of BMMo-derived DCs were significantly decreased in the I/R injured group 
      (compared with the sham-operated group) at 2 d but not 14 d. A comparison of the 
      their functionality found mixed lymphocyte response [MLR] and IL-12 production 
      were similar in the two groups at both time points. Also, immunohistochemistry 
      showed infiltrating DCs in the outer medulla of the I/R injured kidney at 2 d but 
      not 14 d of reperfusion. Thus, I/R stress reduces the number of DCs 
      differentiated from BMMos but not the functional activity of these DCs. This 
      decrease may reflect a stress-induced downshift in the capacity of BMMos to 
      differentiate into DCs and a parallel upshift in the capacity of DCs to 
      infiltrate the kidney.
FAU - Wu, Chih-Jen
AU  - Wu CJ
AD  - Division of Nephrology, Mackay Memorial Hospital, Taipei, Taiwan.
FAU - Sheu, Joen-Rong
AU  - Sheu JR
FAU - Chen, Han-Hsiang
AU  - Chen HH
FAU - Liao, Hui-Fen
AU  - Liao HF
FAU - Yang, Yuh-Cheng
AU  - Yang YC
FAU - Yang, Stone
AU  - Yang S
FAU - Chen, Yu-Jen
AU  - Chen YJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051024
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*physiology/ultrastructure
MH  - Cell Differentiation/physiology
MH  - Dendritic Cells/*physiology/ultrastructure
MH  - Flow Cytometry
MH  - Immunohistochemistry
MH  - Interleukin-12/metabolism
MH  - Kidney/*pathology
MH  - Kidney Function Tests
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/*physiology/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*pathology
EDAT- 2005/10/26 09:00
MHDA- 2006/03/10 09:00
CRDT- 2005/10/26 09:00
PHST- 2005/02/05 00:00 [received]
PHST- 2005/06/21 00:00 [accepted]
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2006/03/10 09:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
AID - S0024-3205(05)00850-7 [pii]
AID - 10.1016/j.lfs.2005.06.043 [doi]
PST - ppublish
SO  - Life Sci. 2006 Feb 2;78(10):1121-8. doi: 10.1016/j.lfs.2005.06.043. Epub 2005 Oct 
      24.