PMID- 16246931
OWN - NLM
STAT- MEDLINE
DCOM- 20051215
LR  - 20171116
IS  - 0022-0345 (Print)
IS  - 0022-0345 (Linking)
VI  - 84
IP  - 11
DP  - 2005 Nov
TI  - Induction of MMP-3 by hyaluronan oligosaccharides in temporomandibular joint 
      chondrocytes.
PG  - 1005-9
AB  - Low-molecular-weight hyaluronan (LMW-HA) is often increased in osteoarthritic 
      joints; however, its biological function in cartilage has not been clarified. We 
      hypothesize that LMW-HA causes the catabolic activation of chondrocytes through 
      its interaction with CD44. Cartilage explants and chondrocytes, derived from 
      bovine temporomandibular joints (TMJ), were examined for matrix loss and the 
      expression of matrix metalloproteinase-3 (MMP-3) following treatment with 
      hyaluronan oligosaccharides (HA(oligos)). Hyaluronan and CD44 were uniformly 
      distributed throughout the fibrous and cartilaginous zones of the TMJ condyle. 
      Treatment of cartilage explants with HA(oligos) resulted in cartilage matrix loss 
      with increased secreted caseinolytic activity. HA(oligos) treatment of TMJ 
      chondrocytes resulted in enhanced MMP-3 expression, whereas wash-out of the 
      HA(oligos) in the middle of the experimental period reduced this induction. These 
      results suggest that HA(oligos) activate chondrocytes, resulting in a substantial 
      enhancement of proteinase expression, and the removal of HA(oligos) by wash-out 
      reverses this catabolic activation.
FAU - Ohno, S
AU  - Ohno S
AD  - Department of Biochemistry, Rush Medical College, Rush University Medical Center, 
      1653 W. Congress Parkway, Chicago, IL 60612, USA.
FAU - Ohno-Nakahara, M
AU  - Ohno-Nakahara M
FAU - Knudson, C B
AU  - Knudson CB
FAU - Knudson, W
AU  - Knudson W
LA  - eng
GR  - P50 AR 39239/AR/NIAMS NIH HHS/United States
GR  - R01 AR 39507/AR/NIAMS NIH HHS/United States
GR  - R01 AR 43384/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Caseins)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Oligosaccharides)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/cytology/enzymology
MH  - Caseins/analysis/metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/*enzymology
MH  - Enzyme Induction
MH  - Hyaluronan Receptors/analysis/physiology
MH  - Hyaluronic Acid/*physiology
MH  - Male
MH  - Mandibular Condyle/cytology/enzymology
MH  - Matrix Metalloproteinase 3/analysis/*biosynthesis
MH  - Oligosaccharides/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Temporomandibular Joint/*cytology/enzymology
EDAT- 2005/10/26 09:00
MHDA- 2005/12/16 09:00
CRDT- 2005/10/26 09:00
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2005/12/16 09:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
AID - 84/11/1005 [pii]
AID - 10.1177/154405910508401107 [doi]
PST - ppublish
SO  - J Dent Res. 2005 Nov;84(11):1005-9. doi: 10.1177/154405910508401107.