PMID- 16247677
OWN - NLM
STAT- MEDLINE
DCOM- 20060117
LR  - 20100921
IS  - 0141-5492 (Print)
IS  - 0141-5492 (Linking)
VI  - 27
IP  - 21
DP  - 2005 Nov
TI  - Production of recombinant human placental variant growth hormone in Pichia 
      pastoris.
PG  - 1695-700
AB  - cDNA encoding mature human placental variant growth hormone (HGH-V) was 
      synthesized by retro-transcription polymerase chain reaction (RT-PCR) from total 
      RNA recovered from human term-placenta and cloned in pBluescript plasmid (pBS) in 
      Escherichia coli. cDNA was subcloned into pPIC9, fusing it to the flanking 
      regulatory sequences of the Pichia pastoris alcohol oxidase 1 gene (AOX1) and 
      finally introduced into the genome of this yeast by homologous recombination. The 
      resulting new recombinant strain produced and secreted, towards the culture 
      medium, mature HGH-V, whose activity was demonstrated in cell culture by the Nb2 
      proliferation assay.
FAU - Palma-Nicolas, Jose Prisco
AU  - Palma-Nicolas JP
AD  - Departamento de Bioquimica, Laboratorio de Biotecnologia, Facultad de Medicina de 
      la Universidad Autonoma de Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre Pequeno, 
      Col. Mitras Centro, 64460 Monterrey, N.L., Mexico.
FAU - Ascacio-Martinez, Jorge A
AU  - Ascacio-Martinez JA
FAU - Revol, Agnes
AU  - Revol A
FAU - Barrera-Saldana, Hugo A
AU  - Barrera-Saldana HA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (GH2 protein, human)
RN  - 0 (Placental Hormones)
RN  - 0 (Recombinant Proteins)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Biological Assay
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Female
MH  - Growth Hormone/*biosynthesis/genetics/pharmacology
MH  - Humans
MH  - Pichia/genetics/*metabolism
MH  - Placental Hormones/*biosynthesis/genetics/pharmacology
MH  - Recombinant Proteins/*biosynthesis/genetics/pharmacokinetics
EDAT- 2005/10/26 09:00
MHDA- 2006/01/18 09:00
CRDT- 2005/10/26 09:00
PHST- 2005/03/11 00:00 [received]
PHST- 2005/08/22 00:00 [accepted]
PHST- 2005/10/26 09:00 [pubmed]
PHST- 2006/01/18 09:00 [medline]
PHST- 2005/10/26 09:00 [entrez]
AID - 10.1007/s10529-005-2733-6 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2005 Nov;27(21):1695-700. doi: 10.1007/s10529-005-2733-6.