PMID- 1624809
OWN - NLM
STAT- MEDLINE
DCOM- 19920811
LR  - 20220227
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 149
IP  - 2
DP  - 1992 Jul 15
TI  - Production of monocyte chemoattractant protein-1 by inflamed synovial tissue and 
      cultured synoviocytes.
PG  - 722-7
AB  - This study analyzes the expression of monocyte chemoattractant protein-1 (MCP-1) 
      by inflamed synovial tissue and defines its regulation in cultured synoviocytes. 
      Synoviocytes from patients with rheumatoid arthritis and osteoarthritis express 
      the 0.7-kb MCP-1 mRNA. Stimulation of synoviocytes with IL-1, TNF-alpha, LPS, 
      platelet-derived growth factor, and transforming growth factor-beta-1, but not 
      with basic fibroblast growth factor causes a marked increase in MCP-1 mRNA 
      levels. Expression of the MCP-1 gene is inducible by activators of the protein 
      kinase A (cAMP) and C (PMA) signal transduction pathways and is differentially 
      regulated by the steroids dexamethasone and retinoic acid. Cultured synoviocytes 
      de novo synthesize 12-, 15-, and 15.2-kDa MCP-1 proteins, which increase after 
      stimulation with IL-1. Synovial tissues from donors without joint disease and 
      from patients with rheumatoid or osteoarthritis were analyzed for MCP-1 mRNA 
      expression by in situ hybridization. In these samples MCP-1 mRNA expressing cells 
      were predominantly found in the sublining cell layers, whereas specimens of 
      normal synovial tissue contained only few positive cells. These results identify 
      synoviocytes as a source of MCP-1. Its expression is controlled by peptide 
      regulatory factors that are known to be present in arthritic joints. Detection of 
      cells producing MCP-1 mRNA in synovial tissues from patients with arthritis shows 
      that this gene is expressed in vivo and suggests that MCP-1 can play a role in 
      recruiting monocytes in joint inflammation.
FAU - Villiger, P M
AU  - Villiger PM
AD  - Sam and Rose Stein Institute for Research on Aging, University of California, San 
      Diego, La Jolla 92093.
FAU - Terkeltaub, R
AU  - Terkeltaub R
FAU - Lotz, M
AU  - Lotz M
LA  - eng
GR  - AG-07996/AG/NIA NIH HHS/United States
GR  - AR-39799/AR/NIAMS NIH HHS/United States
GR  - DK-36702/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Glucocorticoids)
RN  - 0 (Growth Substances)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis/genetics
MH  - Cytokines/pharmacology
MH  - Gene Expression Regulation
MH  - Glucocorticoids/pharmacology
MH  - Growth Substances/pharmacology
MH  - Humans
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - Synovial Membrane/*metabolism
MH  - Synovitis/*metabolism
EDAT- 1992/07/15 00:00
MHDA- 1992/07/15 00:01
CRDT- 1992/07/15 00:00
PHST- 1992/07/15 00:00 [pubmed]
PHST- 1992/07/15 00:01 [medline]
PHST- 1992/07/15 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1992 Jul 15;149(2):722-7.