PMID- 16248243
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 25
IP  - 9
DP  - 2005 Sep
TI  - [Experimental study on effect of baoganning on activity of nuclear transcription 
      factor-kappaB in hepatic stellate cells].
PG  - 804-7
AB  - OBJECTIVE: To investigate the effect of Baoganning (BGN) on activity of nuclear 
      transcription factor-kappaB (NF-kappaB) in hepatic stellate cells (HSCs) and its 
      relevant mechanisms. METHODS: Normal Wistar rats were medicated with BGN 
      decoction by gavage for 7 days to prepare BGN drug-serum. The effect of BGN 
      drug-serum on HSC-T6 growth was measured by MTT assay; phosphorylation level of 
      NF-kappaB inhibiting factor IkappaB at different time after BGN stimulation was 
      detected by Western blotting analysis; and the binding level of NF-kappaB with 
      DNA was measured 30 min after drug-serum stimulation with gel shift assay. 
      RESULTS: BGN could significantly inhibit the HSC-T6 growth and quickly supress 
      the phosphorylation of IkappaB, with the effect reached its peak at 30 min and 
      restored to baseline level 6 h after stimulation, and reduce the binding capacity 
      of NF-kappaB with DNA. CONCLUSION: BGN can inhibit phosphorylation of IkappaB, 
      restrain the activity of NF-kappaB and change the binding level of NF-kappaB with 
      DNA.
FAU - Tan, Yan
AU  - Tan Y
AD  - Department of Traditional Chinese Medicine, Southern Medical University, 
      Guangzhou.
FAU - Lu, Zhi-ping
AU  - Lu ZP
FAU - Ou, Su-neng
AU  - Ou SN
FAU - Zhang, Xufu
AU  - Zhang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (NF-kappa B)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA/drug effects/metabolism
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Female
MH  - Hepatocytes/*cytology/metabolism
MH  - Male
MH  - NF-kappa B/*drug effects/genetics/metabolism
MH  - Phosphorylation/drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
EDAT- 2005/10/27 09:00
MHDA- 2007/12/19 09:00
CRDT- 2005/10/27 09:00
PHST- 2005/10/27 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2005/10/27 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Sep;25(9):804-7.