PMID- 16248248
OWN - NLM
STAT- MEDLINE
DCOM- 20071218
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 25
IP  - 9
DP  - 2005 Sep
TI  - [Study on mechanism of the anti-tumor activity of Acanthopanax gracilistylus].
PG  - 825-8
AB  - OBJECTIVE: To study the mechanism of anti-tumor activity of Acanthopanax 
      gracilistylus extract (Age). METHODS: The tumor cells proliferation was detected 
      by using (3H)-TdR incorporation method, and the effects of Age on cell cycle of 
      tumor cells, retinoblastoma (Rb) protein and cyclin-dependent kinases (Cdk) were 
      analyzed by flow cytometry and Western blotting assay, respectively. RESULTS: It 
      was indicated by cytoactivity test in vitro that Age only had effect in 
      inhibiting the proliferation of tumor cells, it couldn't lead to death of cells. 
      Under action of Age, the proliferation of tumor cells was halted at G0/G1 stage 
      of cell cycle, and showed no direct cytotoxic effect by Age. Age could induce 
      lowering of the expression of Rb, Cdk2 and Cdk4, cause halt of tumor cell 
      proliferation. CONCLUSION: The tumor inhibitory effect of Age is realized by way 
      of regulating the activity of cell cycle controlling enzymes to suspend the 
      proliferation of tumor cells.
FAU - Shan, Bao-en
AU  - Shan BE
AD  - Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang. 
      baoenshan@yahoo.com.cn
FAU - Fu, Xiao-mei
AU  - Fu XM
FAU - Hua, Zheng-xiang
AU  - Hua ZX
FAU - Li, Qiaoxia
AU  - Li Q
FAU - Liang, Wenjie
AU  - Liang W
FAU - Liu, Jiqin
AU  - Liu J
FAU - Zhang, Hua
AU  - Zhang H
FAU - Liu, Gangsan
AU  - Liu G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Retinoblastoma Protein)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 4)
SB  - IM
MH  - Adenocarcinoma/pathology
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cyclin-Dependent Kinase 2/biosynthesis
MH  - Cyclin-Dependent Kinase 4/biosynthesis
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Eleutherococcus/*chemistry
MH  - Humans
MH  - Leukemia, T-Cell/pathology
MH  - Mouth Neoplasms/pathology
MH  - Retinoblastoma Protein/biosynthesis
MH  - Stomach Neoplasms/pathology
MH  - Tumor Cells, Cultured
EDAT- 2005/10/27 09:00
MHDA- 2007/12/19 09:00
CRDT- 2005/10/27 09:00
PHST- 2005/10/27 09:00 [pubmed]
PHST- 2007/12/19 09:00 [medline]
PHST- 2005/10/27 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Sep;25(9):825-8.