PMID- 16249002
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20171116
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 187
IP  - 2
DP  - 2006 Aug
TI  - Pro-atherogenic effect of interleukin-4 in endothelial cells: modulation of 
      oxidative stress, nitric oxide and monocyte chemoattractant protein-1 expression.
PG  - 285-91
AB  - BACKGROUND: Although considered as an anti-inflammatory cytokine, interleukin-4 
      (IL-4) has been shown to be pro-atherogenic in mice models of atherosclerosis. 
      OBJECTIVES: In order to elucidate this paradox, we have investigated the effects 
      of IL-4 on characteristic atherogenic parameters in human umbilical vein 
      endothelial cells (HUVECs): production of reactive oxygen species, expression of 
      monocyte chemoattractant protein-1 (MCP-1) and nitric oxide (NO) bioavailability. 
      RESULTS: Incubation of HUVECs with IL-4 resulted in an increased production of 
      reactive oxygen species and extracellular O(2)(-)(*) measured using fluorogenic 
      probes and Cytochrome c that was inhibited by superoxide dismutase or gp91ds-tat, 
      a selective NADPH oxidase inhibitor. The latter also inhibited IL-4 induced 
      over-expression of MCP-1 mRNA measured by classical and real time RT-PCR. 
      Incubation of HUVECs with IL-4 reduced thrombin-induced NO release, detected by 
      electrochemistry, an effect which was reversed by incubation with superoxide 
      dismutase. Both production of reactive oxygen species and MCP-1 mRNA 
      over-expression induced by IL-4 were fully inhibited by selective inhibitors of 
      phosphatidyl inositol 3-kinase. CONCLUSION: The data demonstrate that IL-4 
      up-regulates the expression of MCP-1 and decreases NO bioavailability through 
      activation of NADPH oxidase in endothelial cells. These results are in favor of a 
      pro-inflammatory and pro-atherogenic effect of IL-4 in vascular tissues.
FAU - Walch, L
AU  - Walch L
AD  - UMR7131 CNRS/Universite Pierre et Marie Curie (Paris 6), Hopital Broussais, 102 
      rue Didot, 75014 Paris, France. laurence.walch@brs.ap-hop-paris.fr
FAU - Massade, L
AU  - Massade L
FAU - Dufilho, M
AU  - Dufilho M
FAU - Brunet, A
AU  - Brunet A
FAU - Rendu, F
AU  - Rendu F
LA  - eng
PT  - Journal Article
DEP - 20051024
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Arachidonic Acids)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (IL4 protein, human)
RN  - 0 (Morpholines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 00XIW1CR0F (arachidonyltrifluoromethane)
RN  - 207137-56-2 (Interleukin-4)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - Arachidonic Acids/pharmacology
MH  - Atherosclerosis/immunology/*metabolism
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Chromones/pharmacology
MH  - Endothelium, Vascular/cytology/drug effects/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Humans
MH  - Interleukin-4/*metabolism/pharmacology
MH  - Morpholines/pharmacology
MH  - NADPH Oxidases/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Oxidative Stress/drug effects/*immunology
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/metabolism/pharmacology
MH  - Umbilical Veins/cytology
EDAT- 2005/10/27 09:00
MHDA- 2006/12/23 09:00
CRDT- 2005/10/27 09:00
PHST- 2005/05/12 00:00 [received]
PHST- 2005/08/31 00:00 [revised]
PHST- 2005/09/20 00:00 [accepted]
PHST- 2005/10/27 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2005/10/27 09:00 [entrez]
AID - S0021-9150(05)00606-4 [pii]
AID - 10.1016/j.atherosclerosis.2005.09.016 [doi]
PST - ppublish
SO  - Atherosclerosis. 2006 Aug;187(2):285-91. doi: 
      10.1016/j.atherosclerosis.2005.09.016. Epub 2005 Oct 24.