PMID- 16250446
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 23
IP  - 4
DP  - 2005 Jul
TI  - [Quantitative analysis of cantide in plasma by capillary gel electrophoresis].
PG  - 374-7
AB  - Cantide is a 20-mer antisense phosphorothioate oligonucleotide that inhibits 
      telomerase catalytic subunit hTERT, pharmacologic results showed that it had 
      promising antitumor activity. In order to study the pharmacokinetic properties of 
      Cantide, a capillary gel electrophoretic (CGE) method with internal standard was 
      used for the determination of Cantide in rat plasma. Cantide and the internal 
      standard had approximately equal percentage of base composition. Extraction of 
      the phosphorothioate oligonucleotides from plasma was accomplished using two 
      solid-phase extraction columns, a strong anion-exchange column to remove plasma 
      proteins and lipids, followed by a reversed-phase column to remove plasma salts. 
      A second desalting step, achieved by dialysis utilizing a membrane, was required 
      to remove residual ionic material from the extracted sample. The size of the 
      capillary column was 31 cm x 100 microm i.d. with an effective length of 20 cm. 
      The running buffer was a mixture of Tris-boric acid-urea (pH 8.5). The 
      calibration curve was linear in the range of 12.5 - 400 mg/L, with correlation 
      coefficient (r) of 0. 999 8. Intra-day and inter-day relative standard deviations 
      (RSDs) for the extracted samples were 0.398% - 2.46% and 2.75% - 6.07%, 
      respectively. The range of recoveries was 99.53% - 102.1%. The results 
      demonstrate the high accuracy, stability and reproducibility of the procedure.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Beijing Institute of Radiation Medicine, Beijing 100850, China. 
      zhangweilu1980@yahoo.com.cn
FAU - Yang, Binghu
AU  - Yang B
FAU - Liang, Qiande
AU  - Liang Q
FAU - Lu, Dandan
AU  - Lu D
FAU - Lin, Ruxian
AU  - Lin R
FAU - Wang, Shengqi
AU  - Wang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Phosphorothioate Oligonucleotides)
RN  - 0 (cantide)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Capillary/*methods
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phosphorothioate Oligonucleotides/*blood
MH  - Rats
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
EDAT- 2005/10/28 09:00
MHDA- 2010/05/07 06:00
CRDT- 2005/10/28 09:00
PHST- 2005/10/28 09:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PHST- 2005/10/28 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2005 Jul;23(4):374-7.