PMID- 16253119
OWN - NLM
STAT- MEDLINE
DCOM- 20051207
LR  - 20061115
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 62
IP  - 4
DP  - 2005 Oct
TI  - Adjuvant effect of synthetic oligodeoxyribonucleotides (CpG-ODN) from the 
      Paracoccidioides brasiliensis gp43 gene on the Th2-Th1 immunomodulation of 
      experimental paracoccidioidomycosis.
PG  - 325-33
AB  - Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides 
      brasiliensis. Immunostimulatory effects of P. brasiliensis DNA and 
      CpG-oligodeoxyribonucleotides (CpG-ODN) have shown a Th2-Th1 immunomodulation of 
      the isogenic murine model of susceptibility, which develops a progressive and 
      disseminating disease. In this study, we investigated the optimum time interval 
      and doses of CpG-ODN which are able to induce Th2-Th1 immunomodulation. The 
      optimum concentrations for the induction of a decrease in antibody production 
      were 0.5 and 1 microg. Mice immunized twice with CpG-ODN and gp43 (5 and 7 days 
      before the challenge) showed a 60% higher chance of survival compared with the 
      control group (nonimmunized), and an increase in Th1 isotype (IgG2a) was also 
      observed. In vitro assays of naive and preimmunized mice showed discrete cellular 
      proliferation when stimulated by suitable concentrations of CpG-ODN. Type 1 
      cytokines interleukin-12 (IL-12) and interferon-gamma were increased in cell 
      culture supernatants, but no significant difference was found in Th2 IL-4 
      cytokines in stimulated or nonstimulated cell cultures. Concerning the Th2-Th1 
      kinetics in experimental PCM models by adjuvant effect of CpG-ODN, there are 
      still many questions to be answered and clarified. However, the gathering of data 
      obtained in this investigation has led us to suggest that the modulation of 
      Th2-Th1 in experimental PCM depends on time and CpG-ODN concentration.
FAU - Amaral, C C
AU  - Amaral CC
AD  - Discipline of Cellular Biology, Department of Microbiology, Immunology and 
      Parasitology, Federal University of Sao Paulo, Brazil.
FAU - Garcia, I P
AU  - Garcia IP
FAU - Fernandes, G F
AU  - Fernandes GF
FAU - Almeida, S R
AU  - Almeida SR
FAU - Camargo, Z P
AU  - Camargo ZP
FAU - Souza, M C
AU  - Souza MC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Animals
MH  - Antibodies, Fungal/biosynthesis/blood
MH  - Antigens, Fungal/*genetics
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dose-Response Relationship, Immunologic
MH  - Fungal Proteins/chemical synthesis/*genetics
MH  - Genes, Fungal/immunology
MH  - Glycoproteins/chemical synthesis/*genetics
MH  - Lymph Nodes/cytology/immunology/metabolism
MH  - Mice
MH  - Oligodeoxyribonucleotides/chemical synthesis/*pharmacology
MH  - Paracoccidioides/*genetics/growth & development/immunology
MH  - Paracoccidioidomycosis/immunology/pathology/*therapy
MH  - Spleen/microbiology/pathology
MH  - Th1 Cells/drug effects/*immunology
MH  - Th2 Cells/drug effects/*immunology
EDAT- 2005/10/29 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/10/29 09:00
PHST- 2005/10/29 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/10/29 09:00 [entrez]
AID - SJI1680 [pii]
AID - 10.1111/j.1365-3083.2005.01680.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2005 Oct;62(4):325-33. doi: 10.1111/j.1365-3083.2005.01680.x.