PMID- 16253226
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20220726
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 524
IP  - 1-3
DP  - 2005 Nov 7
TI  - Vasodilatory and anti-inflammatory effects of the 
      1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG) via a nitric oxide-cGMP pathway.
PG  - 111-9
AB  - Vasorelaxant and anti-inflammatory effects of a 
      1,2,3,4,6-penta-O-galloyl-beta-d-glucose (PGG) isolated from the root barks of 
      Paeonia suffruticosa and possible mechanisms responsible were investigated. PGG 
      induced a concentration-dependent relaxation of the phenylephrine-precontracted 
      rat aorta. This effect disappeared with the removal of functional endothelium. 
      Pretreatment of the aortic tissues with either N(G)-nitro-L-arginine methyl ester 
      (L-NAME) or 1H-[1,2,4]-oxadiazole-[4,3-alpha]-quinoxalin-1-one (ODQ) inhibited 
      the relaxation induced by PGG. Incubation of human umbilical vein endothelial 
      cells (HUVECs) or carotid arteries isolated from rats with PGG increased the 
      production of cGMP in a dose-dependent manner, but this effect was blocked by 
      pretreatment with L-NAME and ODQ, respectively. PGG treatment attenuated tumor 
      necrosis factor-alpha (TNF-alpha)-induced nuclear factor-kappaB (NF-kappaB) p65 
      translocation in human umbilical vein endothelial cells. In addition, PGG 
      suppressed the expression levels of adhesion molecules including intracellular 
      cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) 
      induced by TNF-alpha. TNF-alpha-induced monocyte chemoattractant protein-1 
      (MCP-1) expression was also attenuated by addition of PGG. PGG treatment 
      inhibited cellular adhesion of U937 cells onto human umbilical vein endothelial 
      cells induced by TNF-alpha. Taken together, the present study suggests that PGG 
      dilates vascular smooth muscle and suppresses the vascular inflammatory process 
      via endothelium-dependent nitric oxide (NO)/cGMP signaling.
FAU - Kang, Dae Gill
AU  - Kang DG
AD  - Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, 
      Jeonbuk, Republic of Korea.
FAU - Moon, Mi Kyoung
AU  - Moon MK
FAU - Choi, Deok Ho
AU  - Choi DH
FAU - Lee, Jun Kyoung
AU  - Lee JK
FAU - Kwon, Tae Oh
AU  - Kwon TO
FAU - Lee, Ho Sub
AU  - Lee HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051025
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (NF-kappa B)
RN  - 0 (Oxadiazoles)
RN  - 0 (Quinoxalines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Vasodilator Agents)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3UI3K8W93I (pentagalloylglucose)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - H2D2X058MU (Cyclic GMP)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Aorta, Thoracic/drug effects/physiology
MH  - Blotting, Western
MH  - Carotid Arteries/drug effects/metabolism
MH  - Cell Adhesion/drug effects
MH  - Cell Line
MH  - Chemokine CCL2/genetics
MH  - Cyclic GMP/biosynthesis/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Endothelial Cells/drug effects/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression/drug effects
MH  - Guanylate Cyclase/antagonists & inhibitors
MH  - Humans
MH  - Hydrolyzable Tannins/*pharmacology
MH  - In Vitro Techniques
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Male
MH  - NF-kappa B/metabolism
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/*physiology
MH  - Nitric Oxide Synthase/antagonists & inhibitors
MH  - Oxadiazoles/pharmacology
MH  - Paeonia/chemistry
MH  - Plant Bark/chemistry
MH  - Plant Roots/chemistry
MH  - Quinoxalines/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/*drug effects
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - U937 Cells
MH  - Umbilical Veins/cytology
MH  - Vascular Cell Adhesion Molecule-1/genetics
MH  - Vasodilation/drug effects
MH  - Vasodilator Agents/*pharmacology
EDAT- 2005/10/29 09:00
MHDA- 2006/01/06 09:00
CRDT- 2005/10/29 09:00
PHST- 2005/04/14 00:00 [received]
PHST- 2005/08/18 00:00 [revised]
PHST- 2005/08/29 00:00 [accepted]
PHST- 2005/10/29 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/10/29 09:00 [entrez]
AID - S0014-2999(05)00895-2 [pii]
AID - 10.1016/j.ejphar.2005.08.061 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2005 Nov 7;524(1-3):111-9. doi: 10.1016/j.ejphar.2005.08.061. 
      Epub 2005 Oct 25.