PMID- 16253299
OWN - NLM
STAT- MEDLINE
DCOM- 20060615
LR  - 20061115
IS  - 0034-5288 (Print)
IS  - 0034-5288 (Linking)
VI  - 81
IP  - 1
DP  - 2006 Aug
TI  - Identification of bovine dendritic cell phenotype from bovine peripheral blood.
PG  - 40-5
AB  - Dendritic cells (DCs) are professional antigen presenting cells, which initiate 
      primary immune responses and also play an important role in the generation of 
      peripheral tolerance. There is no reliable method established for the isolation 
      of bovine peripheral blood DCs, and furthermore, the phenotypes and the functions 
      of bovine DCs are still not fully clear. In the present study, we have attempted 
      to identify bovine peripheral blood DCs by negative-selection. In bovine 
      peripheral blood mononuclear cells (PBMC), we have newly characterized the 
      phenotype of DCs, which is CD11c+/CD172a+. These cells display features of 
      myeloid type DCs. In the thymic medulla, CD11c+/CD172a+ cells were also present 
      and CD1+/CD172a+ cells were additionally detected as a population of DCs. The 
      data suggest that one of the bovine DCs phenotypes from PBMC is derived from 
      myeloid lineages lacking a CD1 molecule, which then drift to several tissues, and 
      that they then may express a CD1 molecule upon their functional differentiation.
FAU - Miyazawa, Kohtaro
AU  - Miyazawa K
AD  - Laboratory of Functional Morphology, Graduate School of Agricultural Science, 
      Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555 Sendai, 
      Japan.
FAU - Aso, Hisashi
AU  - Aso H
FAU - Honda, Masashi
AU  - Honda M
FAU - Kido, Taketomo
AU  - Kido T
FAU - Minashima, Takeshi
AU  - Minashima T
FAU - Kanaya, Takashi
AU  - Kanaya T
FAU - Watanabe, Kouichi
AU  - Watanabe K
FAU - Ohwada, Shyuichi
AU  - Ohwada S
FAU - Rose, Michael T
AU  - Rose MT
FAU - Yamaguchi, Takahiro
AU  - Yamaguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051025
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Antigens, CD1)
SB  - IM
MH  - Animals
MH  - Antigens, CD1/*analysis
MH  - Cattle
MH  - Cell Differentiation
MH  - Dendritic Cells/*classification/*immunology
MH  - Leukocytes, Mononuclear
MH  - Phenotype
EDAT- 2005/10/29 09:00
MHDA- 2006/06/16 09:00
CRDT- 2005/10/29 09:00
PHST- 2005/06/17 00:00 [received]
PHST- 2005/08/08 00:00 [revised]
PHST- 2005/09/01 00:00 [accepted]
PHST- 2005/10/29 09:00 [pubmed]
PHST- 2006/06/16 09:00 [medline]
PHST- 2005/10/29 09:00 [entrez]
AID - S0034-5288(05)00165-7 [pii]
AID - 10.1016/j.rvsc.2005.09.003 [doi]
PST - ppublish
SO  - Res Vet Sci. 2006 Aug;81(1):40-5. doi: 10.1016/j.rvsc.2005.09.003. Epub 2005 Oct 
      25.