PMID- 16253445
OWN - NLM
STAT- MEDLINE
DCOM- 20061012
LR  - 20131121
IS  - 0378-4320 (Print)
IS  - 0378-4320 (Linking)
VI  - 95
IP  - 3-4
DP  - 2006 Oct
TI  - Changes in gene expression involved in energy utilization during chicken follicle 
      development.
PG  - 283-94
AB  - Ovarian follicle development in egg-laying species is characterized by rapid 
      growth in 7 days prior to ovulation when DNA and protein synthesis is markedly 
      increased in the granulosa and theca cells. However, energy and substrate sources 
      to facilitate the extensive DNA and protein synthesis necessary for 
      folliculogenesis have not been identified in avian species. The current study was 
      undertaken to investigate the expression profiles of regulatory genes involved in 
      glucose transport, glycolysis and fatty acid oxidation in the follicle membranes 
      from the small white follicle (SWF) to follicle 1 (F1) stages of follicle 
      development. In our analysis of glucose transporter (GLUT) isoform expression, 
      the level of GLUT1 mRNA increased with follicle development while GLUT2, GLUT3 
      and GLUT8 mRNA levels were unaffected by follicle development. In contrast, the 
      expression patterns of proteins involved in metabolism down-stream of glucose 
      transport, including hexokinase (HK), pyruvate dehydrogenase E1alpha (PDH 
      E1alpha) and citrate synthase (CS), did not vary with the developmental stage of 
      the follicle, even during rapid follicle growth. Expression of genes related to 
      beta-oxidation of fatty acids (carnitine palmityl CoA transferase I and II, 
      l-3-hydroxyacyl CoA dehydrogenase and long-chain acyl-CoA dehydrogenase), for 
      which expression in the ovarian follicles of mammalian species has not previously 
      been studied, was not changed consistently with the follicle development. These 
      results suggest that both glucose and fatty acids might work as energy sources to 
      ensure rapid follicle development in the chicken ovary, even though glycolysis 
      and beta-oxidation are not modulated by follicle development.
FAU - Seol, H S
AU  - Seol HS
AD  - Animal Nutrition, Division of Life Sciences, Graduate School of Agricultural 
      Science, Tohoku University, Sendai 981-8555, Japan.
FAU - Sato, Kan
AU  - Sato K
FAU - Murakami, Hitoshi
AU  - Murakami H
FAU - Toyomizu, Masaaki
AU  - Toyomizu M
FAU - Akiba, Yukio
AU  - Akiba Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051025
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Glucose Transport Proteins, Facilitative)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (RNA, Messenger)
RN  - EC 1.2.4.1 (Pyruvate Dehydrogenase (Lipoamide))
RN  - EC 1.2.4.1 (pyruvate dehydrogenase E1alpha subunit)
RN  - EC 1.3.- (Acyl-CoA Dehydrogenases)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Acyl-CoA Dehydrogenases/genetics
MH  - Animals
MH  - Carnitine O-Palmitoyltransferase/genetics
MH  - Chickens/*genetics/physiology
MH  - Citrate (si)-Synthase/genetics
MH  - Energy Metabolism/*genetics
MH  - Female
MH  - Gene Expression/*physiology
MH  - Glucose/metabolism
MH  - Glucose Transport Proteins, Facilitative/genetics
MH  - Glucose Transporter Type 1/genetics
MH  - Hexokinase/genetics
MH  - Lipid Metabolism/genetics
MH  - Ovarian Follicle/*growth & development
MH  - Pyruvate Dehydrogenase (Lipoamide)/genetics
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2005/10/29 09:00
MHDA- 2006/10/13 09:00
CRDT- 2005/10/29 09:00
PHST- 2005/06/08 00:00 [received]
PHST- 2005/09/14 00:00 [revised]
PHST- 2005/09/23 00:00 [accepted]
PHST- 2005/10/29 09:00 [pubmed]
PHST- 2006/10/13 09:00 [medline]
PHST- 2005/10/29 09:00 [entrez]
AID - S0378-4320(05)00278-2 [pii]
AID - 10.1016/j.anireprosci.2005.09.016 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2006 Oct;95(3-4):283-94. doi: 10.1016/j.anireprosci.2005.09.016. 
      Epub 2005 Oct 25.