PMID- 16253529
OWN - NLM
STAT- MEDLINE
DCOM- 20070425
LR  - 20161124
IS  - 1090-0233 (Print)
IS  - 1090-0233 (Linking)
VI  - 173
IP  - 1
DP  - 2007 Jan
TI  - Gastrointestinal parasitism reduces the plasma availability of doramectin in 
      lambs.
PG  - 167-73
AB  - A study was undertaken to investigate the effect of parasitism on plasma 
      availability and pharmacokinetic behaviour of doramectin (DRM) in lambs. Fourteen 
      parasitised grey face Suffolk lambs (26.9 +/- 1.5 kg bodyweight) were selected 
      for the study. Seven pairs of lambs were allocated to two groups to obtain an 
      approximately even weight distribution. Group I (non-parasitised) was pre-treated 
      with three repeated administrations of 5 mg/kg fenbendazole to maintain a 
      parasite free condition. In group II (parasitised), the lambs did not receive any 
      anthelmintic treatment. After the 85-day pre-treatment period, both groups of 
      animals were treated with DRM by subcutaneous (SC) injection in the shoulder area 
      at 200 microg/kg. Throughout the experimental period, both groups were maintained 
      together under similar feeding and management conditions. Blood samples were 
      collected by jugular venepuncture at different set times between 0.5 h and 60 
      days post-treatment. After plasma extraction and derivatisation, samples were 
      analysed by high performance liquid chromatography (HPLC) with fluorescence 
      detection. A computerised kinetic analysis was performed and the data were 
      compared using the Student's paired t test. The parent molecule was detected in 
      plasma between 30 min and either day 20 (parasitised) or day 35 (non-parasitised) 
      post-DRM treatment. The AUC values of the parasitised group (143.0 +/- 18 ng 
      d/mL) were significantly lower (P<0.05) than those observed in the parasitically 
      naive animals (229.6 +/- 21.7 ng d/mL). The mean residence time (MRT) in the 
      parasitised group (3.4 +/- 0.3 days) was significantly shorter (P<0.05) than in 
      the healthy group (6.6 +/- 0.6 days). Study results have shown that parasitic 
      disease, through alteration in the body condition, can produce significant 
      changes in the plasma disposition of DRM when administered SC to parasitised 
      lambs.
FAU - Perez, Ruben
AU  - Perez R
AD  - Laboratorio de Farmacologia, Facultad Medicina Veterinaria, Universidad de 
      Concepcion, P.O. Box 537 Chillan, Chile. rubperez@udec.cl
FAU - Palma, Cristina
AU  - Palma C
FAU - Araneda, Marcia
AU  - Araneda M
FAU - Cabezas, Ignacio
AU  - Cabezas I
FAU - Rubilar, Luis
AU  - Rubilar L
FAU - Arboix, Margarita
AU  - Arboix M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051025
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Anthelmintics)
RN  - 70288-86-7 (Ivermectin)
RN  - KGD7A54H5P (doramectin)
SB  - IM
MH  - Animals
MH  - Anthelmintics/*pharmacokinetics
MH  - Biological Availability
MH  - Feces/parasitology
MH  - Gastrointestinal Diseases/parasitology/*veterinary
MH  - Ivermectin/*analogs & derivatives/pharmacokinetics
MH  - Parasite Egg Count
MH  - Sheep
MH  - Sheep Diseases/drug therapy/*parasitology
EDAT- 2005/10/29 09:00
MHDA- 2007/04/26 09:00
CRDT- 2005/10/29 09:00
PHST- 2005/08/02 00:00 [accepted]
PHST- 2005/10/29 09:00 [pubmed]
PHST- 2007/04/26 09:00 [medline]
PHST- 2005/10/29 09:00 [entrez]
AID - S1090-0233(05)00202-9 [pii]
AID - 10.1016/j.tvjl.2005.08.010 [doi]
PST - ppublish
SO  - Vet J. 2007 Jan;173(1):167-73. doi: 10.1016/j.tvjl.2005.08.010. Epub 2005 Oct 25.