PMID- 16254113
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20181113
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 58
IP  - 11
DP  - 2005 Nov
TI  - Grading melanocytic dysplasia in paraffin wax embedded tissue by the nucleic acid 
      index.
PG  - 1206-10
AB  - BACKGROUND: Although nucleic acid derangements are the hallmark of melanocytic 
      dysplasia, the gold standard for its diagnosis remains the microscopic evaluation 
      of haematoxylin and eosin stained slides. However, light microscopy is subjective 
      and crucial genomic changes do not always show as changes in histology. AIMS: To 
      introduce the nucleic acid index (NAI) as a means of analysing nucleic acid 
      derangements in histological sections at the level of the individual cell and 
      within the context of its microenvironment. METHODS: Confocal laser scanning 
      microscopy was performed on melanocytic lesions stained with acridine orange 
      (AO), a fluorescent stain for DNA and RNA. The NAI, calculated by measuring the 
      fluorescence intensities of AO in nuclei relative to the surrounding cytoplasm, 
      reflects the concentration of DNA relative to RNA. RESULTS: When applied to 
      benign naevi, dysplastic naevi, and melanoma, a very strong significant 
      association was seen between lower NAI and malignant potential (p < 0.0001). 
      Strong inverse correlations were found between NAI and both mitotic index and 
      Breslow thickness. Interestingly, the NAI for dysplastic naevi is between that of 
      melanoma and most benign naevi, consistent with their intermediate biological 
      behaviour and histological appearance. CONCLUSION: By providing a quantitative 
      measure for melanocytic neoplasia, the NAI may improve the diagnosis of 
      melanocytic lesions and the selection of treatment.
FAU - Berman, D M
AU  - Berman DM
AD  - Laboratory of Pathology, National Cancer Institute, Bldg 10-2N212, 10 Center 
      Drive Bethesda, MD 20896, USA. david.berman@bms.com
FAU - Wincovitch, S
AU  - Wincovitch S
FAU - Garfield, S
AU  - Garfield S
FAU - Romeo, M J
AU  - Romeo MJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - DNA, Neoplasm/*analysis
MH  - Diagnosis, Differential
MH  - Dysplastic Nevus Syndrome/*diagnosis/genetics/pathology
MH  - Humans
MH  - Image Processing, Computer-Assisted/methods
MH  - Melanocytes/pathology
MH  - Melanoma/*diagnosis/genetics/pathology
MH  - Microscopy, Confocal/methods
MH  - Mitotic Index
MH  - Nevus, Pigmented/diagnosis/genetics/pathology
MH  - Paraffin Embedding
MH  - RNA, Neoplasm/analysis
MH  - Skin Neoplasms/*diagnosis/genetics/pathology
PMC - PMC1770753
EDAT- 2005/10/29 09:00
MHDA- 2006/01/06 09:00
PMCR- 2008/11/01
CRDT- 2005/10/29 09:00
PHST- 2005/10/29 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/10/29 09:00 [entrez]
PHST- 2008/11/01 00:00 [pmc-release]
AID - 58/11/1206 [pii]
AID - 0581206 [pii]
AID - 10.1136/jcp.2005.026559 [doi]
PST - ppublish
SO  - J Clin Pathol. 2005 Nov;58(11):1206-10. doi: 10.1136/jcp.2005.026559.