PMID- 16254326
OWN - NLM
STAT- MEDLINE
DCOM- 20060127
LR  - 20240412
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 79
IP  - 22
DP  - 2005 Nov
TI  - Expression and mutational analysis of Autographa californica nucleopolyhedrovirus 
      HCF-1: functional requirements for cysteine residues.
PG  - 13900-14
AB  - The host cell-specific factor 1 gene (hcf-1) of the baculovirus Autographa 
      californica multiple nucleopolyhedrovirus is required for efficient virus growth 
      in TN368 cells but is dispensable for virus replication in SF21 cells. However, 
      the mechanism of action of hcf-1 is unknown. To begin to understand its function 
      in virus replication we have investigated the expression and localization pattern 
      of HCF-1 in infected cells. Analysis of virus-infected TN368 cells showed that 
      hcf-1 is expressed at an early time in the virus life cycle, between 2 and 12 h 
      postinfection, and localized the protein to punctate nuclear foci. Through 
      coprecipitation experiments we have confirmed that HCF-1 self-associates into 
      dimers or higher-order structures. We also found that overexpression of HCF-1 
      repressed expression from the hcf-1 promoter in transient reporter assays. 
      Mutagenesis of cysteine residues within a putative RING finger domain in the 
      amino acid sequence of HCF-1 abolished self-association activity and suggests 
      that the RING domain may be involved in this protein-protein interaction. A 
      different but overlapping set of cysteine residues were required for efficient 
      gene repression activity. Functional analysis of HCF-1 mutants showed that the 
      cysteine amino acids required for both self-association and gene repression 
      activities of HCF-1 were also required for efficient late-gene expression and 
      occlusion body formation in TN368 cells. Mutational analysis also identified 
      essential charged and hydrophobic amino acids located between two of the 
      essential cysteine residues. We propose that HCF-1 is a RING finger-containing 
      protein whose activity requires HCF-1 self-association and gene repression 
      activity.
FAU - Wilson, Joyce A
AU  - Wilson JA
AD  - Departments of Entomology, University of Georgia, Athens, Georgia 30602, USA. 
      jwilson@uhnres.utoronto.ca
FAU - Forney, Scott D
AU  - Forney SD
FAU - Ricci, Alessondra M
AU  - Ricci AM
FAU - Allen, Emily G
AU  - Allen EG
FAU - Hefferon, Kathleen L
AU  - Hefferon KL
FAU - Miller, Lois K
AU  - Miller LK
LA  - eng
GR  - AI23719/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA Primers)
RN  - 0 (HCF-1 protein, Autographa californica nucleopolyhedrovirus)
RN  - 0 (Viral Proteins)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Consensus Sequence
MH  - Cysteine
MH  - DNA Primers
MH  - *Gene Expression Regulation, Viral
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nucleopolyhedroviruses/*genetics
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - Protein Engineering
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spodoptera
MH  - Viral Proteins/chemistry/*genetics
PMC - PMC1280185
EDAT- 2005/10/29 09:00
MHDA- 2006/01/28 09:00
PMCR- 2005/11/01
CRDT- 2005/10/29 09:00
PHST- 2005/10/29 09:00 [pubmed]
PHST- 2006/01/28 09:00 [medline]
PHST- 2005/10/29 09:00 [entrez]
PHST- 2005/11/01 00:00 [pmc-release]
AID - 79/22/13900 [pii]
AID - 0593-05 [pii]
AID - 10.1128/JVI.79.22.13900-13914.2005 [doi]
PST - ppublish
SO  - J Virol. 2005 Nov;79(22):13900-14. doi: 10.1128/JVI.79.22.13900-13914.2005.