PMID- 16258512
OWN - NLM
STAT- MEDLINE
DCOM- 20060307
LR  - 20230210
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 19
IP  - 1
DP  - 2006 Jan
TI  - Molecular diagnosis of Ewing sarcoma/primitive neuroectodermal tumor in routinely 
      processed tissue: a comparison of two FISH strategies and RT-PCR in malignant 
      round cell tumors.
PG  - 1-8
AB  - Ewing sarcoma/primitive neuroectodermal tumor (EWS/PNET) is a diagnostically 
      challenging malignant round cell tumor with signature translocations involving 
      the EWS gene. These translocations are detectable with both reverse 
      transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in situ 
      hybridization (FISH) in formalin-fixed paraffin-embedded tissue. However, RT-PCR 
      is less sensitive in formalin-fixed paraffin-embedded than frozen tissue. 
      Similarly, commercial FISH probes have recently become available, but have yet to 
      be rigorously tested in the clinical setting. Therefore, we have compared RT-PCR 
      with FISH using 'home brew' fusion probes for Ewing sarcoma (EWS)-FLI1 and a 
      commercial EWS break apart probe set in 67 archival round cell tumors, including 
      27 EWS/PNETs. Sensitivities and specificities for both FISH assays were 91 and 
      100%, respectively, whereas RT-PCR had a sensitivity of 54% and a specificity of 
      85%. The break apart strategy was easier to interpret than probe fusion approach. 
      We conclude that FISH is a more sensitive and reliable ancillary technique than 
      RT-PCR for the diagnosis of EWS/PNET in formalin-fixed paraffin-embedded tissue, 
      although the latter provides additional information regarding fusion transcript 
      subtype and prognosis. The commercial break apart probe set is both readily 
      available and easy to interpret, making it particularly attractive. Nonetheless, 
      complex round cell tumors often benefit from molecular testing with multiple 
      methods.
FAU - Bridge, Robert S
AU  - Bridge RS
AD  - Department of Pathology and Immunology, Lauren V Ackerman Laboratory of Surgical 
      Pathology, Barnes-Jewish Hospital, Washington University Medical Center, St 
      Louis, MO 63110-1093, USA.
FAU - Rajaram, Veena
AU  - Rajaram V
FAU - Dehner, Louis P
AU  - Dehner LP
FAU - Pfeifer, John D
AU  - Pfeifer JD
FAU - Perry, Arie
AU  - Perry A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (EWS-FLI fusion protein)
RN  - 0 (EWS1-WT1 fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Neoplasms/*diagnosis/genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Neuroectodermal Tumors, Primitive, Peripheral/*diagnosis/genetics
MH  - Oncogene Proteins, Fusion/genetics
MH  - Proto-Oncogene Protein c-fli-1
MH  - RNA-Binding Protein EWS
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Sarcoma, Ewing/*diagnosis/genetics
MH  - Sensitivity and Specificity
MH  - Transcription Factors/genetics
EDAT- 2005/11/01 09:00
MHDA- 2006/03/08 09:00
CRDT- 2005/11/01 09:00
PHST- 2005/11/01 09:00 [pubmed]
PHST- 2006/03/08 09:00 [medline]
PHST- 2005/11/01 09:00 [entrez]
AID - S0893-3952(22)04485-4 [pii]
AID - 10.1038/modpathol.3800486 [doi]
PST - ppublish
SO  - Mod Pathol. 2006 Jan;19(1):1-8. doi: 10.1038/modpathol.3800486.