PMID- 16258633
OWN - NLM
STAT- MEDLINE
DCOM- 20060710
LR  - 20190606
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 38
IP  - 11
DP  - 2005 Nov
TI  - Human T-cell lymphotropic virus type I (HTLV-I) proviral DNA viral load among 
      asymptomatic patients and patients with HTLV-I-associated myelopathy/tropical 
      spastic paraparesis.
PG  - 1643-7
AB  - To evaluate the human T-cell lymphotropic virus type I (HTLV-I) proviral DNA load 
      among asymptomatic HTLV-I-infected carriers and patients with HTLV-I-associated 
      myelopathy/tropical spastic paraparesis (HAM/TSP), real time PCR using TaqMan 
      probes for the pol gene was performed in two million peripheral blood mononuclear 
      cells (PBMC). The albumin gene was the internal genomic control and MT2 cells 
      were used as positive control. The results are reported as copies/10,000 PBMC, 
      and the detection limit was 10 copies. A total of 89 subjects (44 HAM/TSP and 45 
      healthy HTLV-I-infected carriers) followed up at the Institute of Infectious 
      Diseases "Emilio Ribas" and in the Neurology Division of Hospital of Clinicas 
      were studied. The asymptomatic HTLV-I-infected carriers had a median number of 
      271 copies (ranging from 5 to 4756 copies), whereas the HAM/TSP cases presented a 
      median of 679 copies (5-5360 copies) in 10,000 PBMC. Thus, HAM/TSP patients 
      presented a significantly higher HTLV-I proviral DNA load than healthy HTLV-I 
      carriers (P = 0.005, one-way Mann-Whitney test). As observed in other persistent 
      infections, proviral DNA load quantification may be an important tool for 
      monotoring HTLV-I-infected subjects. However, long-term follow-up is necessary to 
      validate this assay in the clinical setting.
FAU - Montanheiro, P A
AU  - Montanheiro PA
AD  - Departamento de Dermatologia, Hospital das Clinicas, Faculdade de Medicina, 
      Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Oliveira, A C Penalva de
AU  - Oliveira AC
FAU - Posada-Vergara, M P
AU  - Posada-Vergara MP
FAU - Milagres, A C
AU  - Milagres AC
FAU - Tauil, C
AU  - Tauil C
FAU - Marchiori, P E
AU  - Marchiori PE
FAU - Duarte, A J S
AU  - Duarte AJ
FAU - Casseb, J
AU  - Casseb J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051026
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (DNA, Viral)
SB  - IM
EIN - Braz J Med Biol Res. 2005 Dec;38(12):1890. Montanheito, PA [corrected to 
      Montanheiro, PA]
MH  - Case-Control Studies
MH  - DNA, Viral/*analysis/genetics/immunology
MH  - Human T-lymphotropic virus 1/*genetics/immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Paraparesis, Tropical Spastic/immunology/*virology
MH  - Polymerase Chain Reaction
MH  - Proviruses/*genetics/immunology
MH  - Viral Load
EDAT- 2005/11/01 09:00
MHDA- 2006/07/13 09:00
CRDT- 2005/11/01 09:00
PHST- 2005/11/01 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2005/11/01 09:00 [entrez]
AID - S0100-879X2005001100011 [pii]
AID - 10.1590/s0100-879x2005001100011 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2005 Nov;38(11):1643-7. doi: 
      10.1590/s0100-879x2005001100011. Epub 2005 Oct 26.