PMID- 16259615 OWN - NLM STAT- MEDLINE DCOM- 20051222 LR - 20081121 IS - 1076-3279 (Print) IS - 1076-3279 (Linking) VI - 11 IP - 9-10 DP - 2005 Sep-Oct TI - Brain-derived neurotrophic factor enhances periodontal tissue regeneration. PG - 1618-29 AB - To address whether brain-derived neurotrophic factor (BDNF) could be involved in periodontal tissue regeneration, we examined the effects of BDNF on proliferation and the expression of bone (cementum)- related proteins (osteopontin, bone morphogenetic protein [BMP]-2, type I collagen, alkaline phosphatase [ALPase], and osteocalcin) in cultures of human periodontal ligament (HPL) cells, which are thought to be prerequisite for periodontal tissue regeneration, and on proliferation and angiogenesis in human endothelial cells. Furthermore, we examined the effect of BDNF on the regeneration of periodontal tissues in experimentally induced periodontal defects in dogs. BDNF elevated the expression of ALPase and osteocalcin mRNAs and increased the synthesis of osteopontin, BMP-2, and type I collagen DNA in HPL cells. BDNF stimulated mRNA expression of vascular endothelial growth factor-B and tenascin-X, and proliferation and angiogenesis in human endothelial cells. In vivo studies showed that BDNF stimulated the formation of new alveolar bone cementum and connective new fibers, which were inserted into the newly formed cementum and bone. BDNF also stimulated blood capillary formation. These findings suggest that the regulation of functioning of periodontal ligament cells and endothelial cells by BDNF results in the promotion of periodontal tissue regeneration. FAU - Takeda, Katsuhiro AU - Takeda K AD - Department of Periodontal Medicine, Division of Frontier Medical Science, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan. FAU - Shiba, Hideki AU - Shiba H FAU - Mizuno, Noriyoshi AU - Mizuno N FAU - Hasegawa, Naohiko AU - Hasegawa N FAU - Mouri, Yoshihiro AU - Mouri Y FAU - Hirachi, Akio AU - Hirachi A FAU - Yoshino, Hiroshi AU - Yoshino H FAU - Kawaguchi, Hiroyuki AU - Kawaguchi H FAU - Kurihara, Hidemi AU - Kurihara H LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Tissue Eng JT - Tissue engineering JID - 9505538 RN - 0 (BMP2 protein, human) RN - 0 (Bone Morphogenetic Protein 2) RN - 0 (Bone Morphogenetic Proteins) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Collagen Type I) RN - 0 (Procollagen) RN - 0 (RNA, Messenger) RN - 0 (SPP1 protein, human) RN - 0 (Sialoglycoproteins) RN - 0 (Tenascin) RN - 0 (Transforming Growth Factor beta) RN - 0 (tenascin X) RN - 104982-03-8 (Osteocalcin) RN - 106441-73-0 (Osteopontin) RN - 9007-49-2 (DNA) RN - EC 3.1.3.1 (Alkaline Phosphatase) SB - IM MH - Alkaline Phosphatase/metabolism MH - Animals MH - Bone Morphogenetic Protein 2 MH - Bone Morphogenetic Proteins/analysis/metabolism MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Cell Proliferation/drug effects MH - Cells, Cultured MH - Coculture Techniques MH - Collagen Type I/metabolism MH - DNA/biosynthesis MH - Dental Cementum/cytology/drug effects/enzymology/metabolism MH - Dogs MH - Dose-Response Relationship, Drug MH - Endothelium, Vascular/cytology/physiology MH - Female MH - Fibroblasts/cytology/metabolism MH - Fluorescent Antibody Technique, Indirect MH - Gene Expression Regulation/drug effects MH - Humans MH - Immunohistochemistry MH - Neovascularization, Physiologic/drug effects MH - Organ Culture Techniques MH - Osteocalcin/metabolism MH - Osteopontin MH - Periodontal Ligament/*cytology/drug effects/*physiology MH - Procollagen/analysis/metabolism MH - RNA, Messenger/metabolism MH - Regeneration/*drug effects MH - Sialoglycoproteins/analysis/metabolism MH - Skin/cytology MH - Tenascin/metabolism MH - Time Factors MH - Transforming Growth Factor beta/analysis/metabolism MH - Umbilical Veins/cytology EDAT- 2005/11/02 09:00 MHDA- 2005/12/24 09:00 CRDT- 2005/11/02 09:00 PHST- 2005/11/02 09:00 [pubmed] PHST- 2005/12/24 09:00 [medline] PHST- 2005/11/02 09:00 [entrez] AID - 10.1089/ten.2005.11.1618 [doi] PST - ppublish SO - Tissue Eng. 2005 Sep-Oct;11(9-10):1618-29. doi: 10.1089/ten.2005.11.1618.