PMID- 16260063
OWN - NLM
STAT- MEDLINE
DCOM- 20060227
LR  - 20061115
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 142
IP  - 1
DP  - 2005 Dec 7
TI  - Expressions of cytokines and chemokines in the dorsal motor nucleus of the vagus 
      nerve after right vagotomy.
PG  - 47-57
AB  - The aim of this study was to investigate the expression of cytokines, tumor 
      necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1beta), interleukin-6 
      (IL-6) and transforming growth factor-beta 1 (TGF-beta1) and chemokines, 
      fractalkine, monocyte chemoattractant protein 1 (MCP-1) and stromal cell-derived 
      factor 1 (SDF-1) in the dorsal motor nucleus of the vagus nerve (DMV) after right 
      vagotomy. Results showed that the immunoreactivities of IL-1beta, IL-6, 
      TGF-beta1, fractalkine and MCP-1 were upregulated in the DMV at 14 days and the 
      upregulation persisted at least until 28 days following right vagotomy. 
      Quantification analysis revealed significant increases in the number of their 
      immunopositive cells in the right DMV at 14 and 28 days after right vagotomy. 
      Moreover, the upregulation of TNF-alpha immunoreactivity and significantly 
      increased number of TNF-alpha-immunopositive cells were observed in the injured 
      DMV at 7 and 14 days, and the increase in SDF-1-immunopositive cells at 14 days, 
      after right vagotomy. Real time RT-PCR analysis showed the significant increase 
      in the mRNA expression of IL-1beta, fractalkine and MCP-1 at 7 days, and the 
      upregulation of TNF-alpha mRNA expression at 1 day after vagotomy. However, the 
      peak increase in TGF-beta1 mRNA expression was observed at 1 day and the 
      significant increase persisted at least until 14 days following right vagotomy. 
      Double immunofluorescence analysis showed co-localization of lectin, a marker for 
      microglia with CX3CR1 but not with IL-1beta at 14 days following right vagotomy. 
      This study suggests that cytokines and chemokines involved in neuroprotection and 
      neurodestruction could be activated in the axotomized DMV. However, it warrants 
      further investigation to understand the neurodestructive and neuroprotective 
      mechanisms that determine the fate of the vagal motoneurons after vagotomy.
FAU - Ji, Jun Feng
AU  - Ji JF
AD  - Department of Anatomy, Faculty of Medicine, Yong Loo Lin School of Medicine, 
      National University of Singapore, Blk. MD10, 4 Medical Drive, Singapore 117597, 
      Singapore.
FAU - Dheen, S Thameem
AU  - Dheen ST
FAU - Kumar, S Dinesh
AU  - Kumar SD
FAU - He, Bei Ping
AU  - He BP
FAU - Tay, Samuel Sam Wah
AU  - Tay SS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051102
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (1-phenyl-1,4-epoxy-1H,4H-naphtho(1,8-de)(1,2)dioxepin)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Lectins)
RN  - 0 (Naphthalenes)
RN  - 0 (Oxepins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cell Count/methods
MH  - Chemokines/genetics/*metabolism
MH  - Cytokines/genetics/*metabolism
MH  - Functional Laterality/physiology
MH  - Gene Expression Regulation/*physiology
MH  - Immunohistochemistry/methods
MH  - Lectins/metabolism
MH  - Male
MH  - Medulla Oblongata/*metabolism
MH  - Naphthalenes
MH  - Oxepins
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Time Factors
MH  - Vagotomy/methods
EDAT- 2005/11/02 09:00
MHDA- 2006/02/28 09:00
CRDT- 2005/11/02 09:00
PHST- 2005/06/01 00:00 [received]
PHST- 2005/09/07 00:00 [revised]
PHST- 2005/09/16 00:00 [accepted]
PHST- 2005/11/02 09:00 [pubmed]
PHST- 2006/02/28 09:00 [medline]
PHST- 2005/11/02 09:00 [entrez]
AID - S0169-328X(05)00369-4 [pii]
AID - 10.1016/j.molbrainres.2005.09.017 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2005 Dec 7;142(1):47-57. doi: 
      10.1016/j.molbrainres.2005.09.017. Epub 2005 Nov 2.