PMID- 16260295
OWN - NLM
STAT- MEDLINE
DCOM- 20051202
LR  - 20131121
IS  - 0076-6879 (Print)
IS  - 0076-6879 (Linking)
VI  - 397
DP  - 2005
TI  - Advances in microscopy: microautoradiography of single cells.
PG  - 237-56
AB  - Microautoradiography (MAR) is an efficient method to obtain reliable information 
      about the ecophysiology of microorganisms at the single cell level in mixed 
      communities. Data obtained by the traditional MAR method can now be improved 
      significantly when MAR is combined with fluorescence in situ hybridization (FISH) 
      with oligonucleotide probes for the identification of target organisms. This 
      chapter discusses how to use MAR-FISH in various ecosystems with emphasis on the 
      type of information to be obtained, incubation conditions, and detailed protocols 
      for the MAR technique. It also discusses new MAR applications and the type of 
      information that can be obtained, e.g., the use of dual labeling to investigate 
      the simultaneous uptake of several substrates by individual prokaryotes.
FAU - Nielsen, Jeppe Lund
AU  - Nielsen JL
AD  - Department of Life Sciences, Aalborg University, Denmark.
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Propionates)
RN  - 0 (Sewage)
RN  - 10028-17-8 (Tritium)
RN  - 142M471B3J (Carbon Dioxide)
RN  - Q40Q9N063P (Acetic Acid)
SB  - IM
MH  - Acetic Acid/metabolism
MH  - Autoradiography/*methods
MH  - Biodegradation, Environmental
MH  - Biomass
MH  - Carbon Dioxide/metabolism
MH  - Carbon Radioisotopes
MH  - Ecosystem
MH  - In Situ Hybridization, Fluorescence
MH  - Isotope Labeling
MH  - Microscopy/methods
MH  - Microscopy, Confocal/methods
MH  - Propionates/metabolism
MH  - Sewage/microbiology
MH  - Tritium
EDAT- 2005/11/02 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/11/02 09:00
PHST- 2005/11/02 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/11/02 09:00 [entrez]
AID - S0076-6879(05)97014-6 [pii]
AID - 10.1016/S0076-6879(05)97014-6 [doi]
PST - ppublish
SO  - Methods Enzymol. 2005;397:237-56. doi: 10.1016/S0076-6879(05)97014-6.