PMID- 16261432
OWN - NLM
STAT- MEDLINE
DCOM- 20060410
LR  - 20181113
IS  - 0944-1174 (Print)
IS  - 0944-1174 (Linking)
VI  - 40
IP  - 10
DP  - 2005 Oct
TI  - Identification and characterization of novel gut-associated lymphoid tissues in 
      rat small intestine.
PG  - 956-63
AB  - BACKGROUND: The crypt lamina propria of the mouse small intestine has been shown 
      to harbor multiple tiny clusters filled with c-kit- and interleukin 7 receptor 
      (IL-7R)-positive lympho-hemopoietic cells (cryptopatches; CPs). However, it has 
      remained an open question whether similar lymphoid tissue are present in the 
      gastrointesitinal tract in other animals. In the present study, we investigated 
      whether the small intestine of rats harbored lymphoid tissues similar to mouse 
      CPs. METHODS: Immunohistochemical and flow cytometric analyses were carried out 
      using various antibodies, including those to c-kit and IL-7R molecules. RESULTS: 
      Lymphocyte-filled villi (LFVs), populated predominantly with c-kit- and IL-7 
      receptor (IL-7R)-positive cells and less with T cell receptor (TCR)-alphabeta T 
      cells were found throughout the small intestine of young adult rats. Although 
      LFVs were absent from fetal rat intestine, they were first detected at around 2 
      weeks after birth. Notably, in most LFVs that settled in the antimesenteric wall 
      of the small intestine in young adult rats, immunoglobulin M-positive B cells 
      were also detectable at the bottom of the LFVs. In aged rats, lymphocytes in some 
      LFVs displayed a different phenotype, comprising a large B-cell area that 
      included a germinal center. Thus, these clusters represent the first description 
      of isolated lymphoid follicles (ILFs) in the rat small intestine. CONCLUSIONS: 
      The present study provides the first evidence for c-kit- and IL-7R-positive 
      lymphocyte clusters in the rat small intestine. Our data also indicating that 
      LFVs and ILFs may constitute novel organized gut-associated lymphoid tissues in 
      lamina propria of the rat small intestine.
FAU - Hitotsumatsu, Osamu
AU  - Hitotsumatsu O
AD  - Department of Microbiology and Immunology, School of Medicine, Keio University, 
      Tokyo, Japan.
FAU - Hamada, Hiromasa
AU  - Hamada H
FAU - Naganuma, Makoto
AU  - Naganuma M
FAU - Inoue, Nagamu
AU  - Inoue N
FAU - Ishii, Hiromasa
AU  - Ishii H
FAU - Hibi, Toshifumi
AU  - Hibi T
FAU - Ishikawa, Hiromichi
AU  - Ishikawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Gastroenterol
JT  - Journal of gastroenterology
JID - 9430794
SB  - IM
MH  - Animals
MH  - Intestine, Small/*anatomy & histology
MH  - Lymphoid Tissue/*anatomy & histology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2005/11/02 09:00
MHDA- 2006/04/11 09:00
CRDT- 2005/11/02 09:00
PHST- 2005/04/15 00:00 [received]
PHST- 2005/05/26 00:00 [accepted]
PHST- 2005/11/02 09:00 [pubmed]
PHST- 2006/04/11 09:00 [medline]
PHST- 2005/11/02 09:00 [entrez]
AID - 10.1007/s00535-005-1679-8 [doi]
PST - ppublish
SO  - J Gastroenterol. 2005 Oct;40(10):956-63. doi: 10.1007/s00535-005-1679-8.