PMID- 16261643
OWN - NLM
STAT- MEDLINE
DCOM- 20060103
LR  - 20061115
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 19
IP  - 23
DP  - 2005
TI  - Characterization and quantification of soy isoflavone metabolites in serum of 
      renal transplanted patients by high-performance liquid 
      chromatography/electrospray ionization mass spectrometry.
PG  - 3473-81
AB  - During a dietary intervention study on 16 renal transplanted patients, in which 
      25 g/day of animal proteins were replaced with 25 g of soy proteins, the 
      metabolic profile of soy isoflavones in serum was characterized. This paper 
      describes a reliable and fast liquid chromatography/electrospray ionization mass 
      spectrometry (LC/ESI-MS) method, in negative ion mode, allowing the 
      characterization and simultaneous quantification of several soy isoflavone 
      metabolites. Six metabolites were identified and quantified: daidzein ([M-H](-) 
      at m/z 252.8), dihydrodaidzein (DHD, [M-H](-) at m/z 254.8), equol ([M-H](-) at 
      m/z 240.9), O-desmethylangolensin (O-DMA, [M-H](-) at m/z 256.8), genistein 
      ([M-H](-) at m/z 268.8), and dihydrogenistein (DHG, ([M-H(+)](-) at m/z 270.8). 
      Quantification was assessed using two deuterated internal standards, 
      D(3)-daidzein and D(4)-genistein. This method permitted a limit of quantification 
      (LOQ, S/N = 10) and a limit of detection (LOD, S/N = 3) of 0.05 microM and 0.005 
      microM for all analytes, except for genistein, where the LOQ and LOD were 0.005 
      microM and 0.001 microM, respectively. The linearity ranges were from 0.005 to 
      1.5 microM for genistein, from 0.05 to 1.5 microM for DHG, and from 0.05 to 0.7 
      microM for the other metabolites. The relative standard deviations (RSDs) were 
      between 0.19% and 13.9% at the LOQ concentration for all metabolites, and between 
      0.6% and 4.8% at the maximum concentration. On the basis of the results obtained 
      in the dietary intervention study, it was possible to split the patients into 
      five groups characterized by different metabolic pathways.
FAU - Locati, Daniela
AU  - Locati D
AD  - Department of Agri-Food Molecular Sciences, Laboratory of Food Chemistry and Mass 
      Spectrometry, University of Milan, Via Celoria 2, 20133 Milan, Italy. 
      daniel.locati@unimi.it
FAU - Morandi, Sheila
AU  - Morandi S
FAU - Cupisti, Adamasco
AU  - Cupisti A
FAU - Ghiadoni, Lorenzo
AU  - Ghiadoni L
FAU - Arnoldi, Anna
AU  - Arnoldi A
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Isoflavones)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromatography, High Pressure Liquid
MH  - Diet Therapy
MH  - Female
MH  - Humans
MH  - Isoflavones/*blood/pharmacokinetics
MH  - *Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - *Soy Foods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2005/11/02 09:00
MHDA- 2006/01/04 09:00
CRDT- 2005/11/02 09:00
PHST- 2005/11/02 09:00 [pubmed]
PHST- 2006/01/04 09:00 [medline]
PHST- 2005/11/02 09:00 [entrez]
AID - 10.1002/rcm.2222 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2005;19(23):3473-81. doi: 10.1002/rcm.2222.