PMID- 16262655
OWN - NLM
STAT- MEDLINE
DCOM- 20060117
LR  - 20061115
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 22
IP  - 9
DP  - 2005 Nov
TI  - Opposite impacts of tenascin-C and tenascin-R deficiency in mice on the 
      functional outcome of facial nerve repair.
PG  - 2171-9
AB  - The glycoproteins tenascin-C (TNC) and tenascin-R (TNR) are extracellular matrix 
      proteins involved in the development, plasticity and repair of the nervous 
      system. Altered expression patterns after nerve lesions in adult animals have 
      suggested that these molecules influence axonal regeneration. To test this 
      hypothesis, we investigated adult mice constitutively deficient in the expression 
      of TNC, TNR or both, using the facial nerve injury paradigm. Quantitative 
      analysis of vibrissal movements prior to nerve transection and repair 
      (facial-facial anastomosis) did not reveal genotype-specific differences, and 
      thus impacts of the mutations on motor function in intact animals. Two months 
      after nerve repair, recovery of vibrissal whisking was poor in wild-type mice, a 
      typical finding after facial-facial anastomosis in rodents. Differential effects 
      of the mutations on whisking were found: recovery of function was worse in 
      TNC-deficient and better in TNR null mice compared with wild-type littermates. In 
      double-knockout animals, vibrissal performance was insufficient, but to a lesser 
      extent compared with TNC null mutant mice. Retrograde labelling of motoneurons in 
      the same animals showed that similar numbers of motoneurons had reinnervated the 
      whisker pads in all experimental groups precluding varying extents of motoneuron 
      death and/or axon regeneration failures as causes for the different outcomes of 
      nerve repair. Our results provide strong evidence that TNC promotes and TNR 
      impedes recovery after nerve lesion. These findings are of particular interest 
      with regard to the scanty knowledge about factors determining success of 
      regeneration in the peripheral nervous system of mammals.
FAU - Guntinas-Lichius, Orlando
AU  - Guntinas-Lichius O
AD  - Department of Oto-Rhino-Laryngology, University of Cologne, D-50924 Cologne, 
      Germany.
FAU - Angelov, Doychin N
AU  - Angelov DN
FAU - Morellini, Fabio
AU  - Morellini F
FAU - Lenzen, Mithra
AU  - Lenzen M
FAU - Skouras, Emmanouil
AU  - Skouras E
FAU - Schachner, Melitta
AU  - Schachner M
FAU - Irintchev, Andrey
AU  - Irintchev A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Stilbamidines)
RN  - 0 (Tenascin)
RN  - 147604-77-1 (tenascin R)
SB  - IM
MH  - Anastomosis, Surgical/*methods
MH  - Animals
MH  - Cell Count/methods
MH  - Cell Death/physiology
MH  - Disease Models, Animal
MH  - Facial Nerve Injuries/metabolism/*physiopathology/*surgery
MH  - Mice
MH  - Mice, Knockout
MH  - Motor Neurons/metabolism/pathology
MH  - Muscle, Skeletal/pathology
MH  - Nerve Regeneration/physiology
MH  - Recovery of Function/*physiology
MH  - Stilbamidines/metabolism
MH  - Tenascin/*deficiency
MH  - Tibial Nerve/pathology
MH  - Vibrissae/immunology/physiology
EDAT- 2005/11/03 09:00
MHDA- 2006/01/18 09:00
CRDT- 2005/11/03 09:00
PHST- 2005/11/03 09:00 [pubmed]
PHST- 2006/01/18 09:00 [medline]
PHST- 2005/11/03 09:00 [entrez]
AID - EJN4424 [pii]
AID - 10.1111/j.1460-9568.2005.04424.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2005 Nov;22(9):2171-9. doi: 10.1111/j.1460-9568.2005.04424.x.