PMID- 16263136
OWN - NLM
STAT- MEDLINE
DCOM- 20060331
LR  - 20131121
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 78
IP  - 15
DP  - 2006 Mar 6
TI  - Neuronal expression of nuclear transcription factor MafG in the rat medulla 
      oblongata after baroreceptor stimulation.
PG  - 1760-6
AB  - The medulla oblongata is the site of central baroreceptive neurons in mammals. 
      These neurons express specific basic-leucine zipper transcription factors (bZIP) 
      after baroreceptor stimulation. Previously we showed that activation of 
      baroreceptors induced expression of nuclear transcription factors c-Fos and FosB 
      in central baroreceptive neurons. Here we studied the effects of baroreceptor 
      stimulation on induction of MafG, a member of small Maf protein family that 
      functions as dimeric partners for various bZIP transcription factors by forming 
      transcription-regulating complexes, in the rat medulla oblongata. To determine 
      whether gene expression of MafG is induced by stimulation of arterial 
      baroreceptors, we examined the expression of its mRNA by semi-quantitative 
      reverse transcription-PCR method and its gene product by immunohistochemistry. We 
      found that the number of MafG transcripts increased significantly in the medulla 
      oblongata after baroreceptor stimulation. MafG-immunoreactive neurons were 
      distributed in the nucleus tractus solitarii, the dorsal motor nucleus of the 
      vagus nerve, the ambiguous nucleus and the ventrolateral medulla. The numbers of 
      MafG-immunoreactive neurons in these nuclei were significantly greater in test 
      rats than in saline-injected control rats. We also found approximately 20% of 
      MafG-immunoreactive neurons coexpress FosB after baroreceptor stimulation. Our 
      results suggest that MafG cooperates with FosB to play critical roles as an 
      immediate early gene in the signal transduction of cardiovascular regulation 
      mediated by baroreceptive signals in the medulla oblongata.
FAU - Kumaki, Iku
AU  - Kumaki I
AD  - Department of Laboratory Sciences, Gunma University School of Health Sciences, 
      3-39-15 Showa-machi, Maebashi-shi 371-8514, Japan.
FAU - Yang, Dawei
AU  - Yang D
FAU - Koibuchi, Noriyuki
AU  - Koibuchi N
FAU - Takayama, Kiyoshige
AU  - Takayama K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051102
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Fosb protein, rat)
RN  - 0 (MafG Transcription Factor)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 1WS297W6MV (Phenylephrine)
SB  - IM
MH  - Animals
MH  - Gene Expression/*drug effects
MH  - MafG Transcription Factor/*biosynthesis/genetics
MH  - Male
MH  - Medulla Oblongata/cytology/drug effects/*metabolism
MH  - Neurons/drug effects/*metabolism
MH  - Phenylephrine/pharmacology
MH  - Pressoreceptors/*metabolism
MH  - Proto-Oncogene Proteins c-fos/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2005/11/03 09:00
MHDA- 2006/04/01 09:00
CRDT- 2005/11/03 09:00
PHST- 2005/03/01 00:00 [received]
PHST- 2005/08/11 00:00 [accepted]
PHST- 2005/11/03 09:00 [pubmed]
PHST- 2006/04/01 09:00 [medline]
PHST- 2005/11/03 09:00 [entrez]
AID - S0024-3205(05)00995-1 [pii]
AID - 10.1016/j.lfs.2005.08.030 [doi]
PST - ppublish
SO  - Life Sci. 2006 Mar 6;78(15):1760-6. doi: 10.1016/j.lfs.2005.08.030. Epub 2005 Nov 
      2.