PMID- 16265652
OWN - NLM
STAT- MEDLINE
DCOM- 20060706
LR  - 20220408
IS  - 1043-3074 (Print)
IS  - 1043-3074 (Linking)
VI  - 28
IP  - 1
DP  - 2006 Jan
TI  - Metalloproteinases and their inhibitors: influence on tumor invasiveness and 
      metastasis formation in head and neck squamous cell carcinomas.
PG  - 31-9
AB  - BACKGROUND: Matrix metalloproteinases (MMPs) play an important role in tumor 
      invasiveness. This study investigates the expression status of MMPs and tissue 
      inhibitors of metalloproteinases (TIMPs) in head and neck squamous cell 
      carcinomas (HNSCC). METHODS: Of 48 laryngeal squamous cell carcinoma (LSCC) 
      biopsies and 10 HNSCC cell lines, mRNA was isolated, reversely transcribed, and 
      subjected to polymerase chain reaction (PCR) amplifying MMP-1, MMP-2, MMP-9, 
      MMP-10, TIMP-1, and TIMP-2. Silver nitrate-stained gel electrophoresis 
      demonstrated MMP and TIMP expression status. Exemplary immunohistochemistry and 
      zymography confirmed translation and enzyme activity. RESULTS: Densitometric 
      analysis revealed MMP-2 expression and lymph node metastases to be positively and 
      TIMP-1 and TIMP-2 to be negatively correlated with lymph node metastases. TIMP-2 
      expression and tumor size were negatively correlated. MMP-1, MMP-9, and MMP-10 
      expression were not correlated to metastasis formation or tumor size. 
      CONCLUSIONS: Our results suggest that MMP-2 expression enhances, whereas TIMP-1 
      and TIMP-2 both suppress, cancer spread in LSCC.
CI  - (c) 2005 Wiley Periodicals, Inc. Head Neck 27: XXX-XXX, 2005.
FAU - Gorogh, Tibor
AU  - Gorogh T
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, University of 
      Schleswig-Holstein, Campus Kiel, Arnold-Heller-Str. 14, 24105 Kiel, Germany. 
      gorogh@hno.uni-kiel.de
FAU - Beier, Ulf H
AU  - Beier UH
FAU - Baumken, Jens
AU  - Baumken J
FAU - Meyer, Jens E
AU  - Meyer JE
FAU - Hoffmann, Markus
AU  - Hoffmann M
FAU - Gottschlich, Stefan
AU  - Gottschlich S
FAU - Maune, Steffen
AU  - Maune S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (DNA Primers)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Carcinoma, Squamous Cell/enzymology/*pathology
MH  - Cell Line
MH  - DNA Primers
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/enzymology/*pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/*metabolism
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics/metabolism
MH  - Tumor Cells, Cultured
EDAT- 2005/11/03 09:00
MHDA- 2006/07/11 09:00
CRDT- 2005/11/03 09:00
PHST- 2005/11/03 09:00 [pubmed]
PHST- 2006/07/11 09:00 [medline]
PHST- 2005/11/03 09:00 [entrez]
AID - 10.1002/hed.20298 [doi]
PST - ppublish
SO  - Head Neck. 2006 Jan;28(1):31-9. doi: 10.1002/hed.20298.