PMID- 16269197
OWN - NLM
STAT- MEDLINE
DCOM- 20060516
LR  - 20200423
IS  - 0168-1702 (Print)
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 115
IP  - 2
DP  - 2006 Feb
TI  - Evolution of ORF5 of Spanish porcine reproductive and respiratory syndrome virus 
      strains from 1991 to 2005.
PG  - 198-206
AB  - ORF5 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) 
      were analysed to determine genetic diversity, codon usage, positive and negative 
      selection sites and potential changes in the predicted glycoprotein 5 (GP5). A 
      hypothetical GP5 containing all selected sites was constructed to determine its 
      characteristics. These sequences corresponded to isolates obtained 10 years apart 
      (1991-1995, 18 strains) and a second set (n = 46) from 2000 to 2005. Similarity 
      to Lelystad virus (LV) decreased from 95.5% in 1991-1995 to 89.5% in 2000-2005. 
      Three highly variable regions were found in ORF5. Codon usage was different in 
      both sets for leucine, glutamine, serine and proline. Thus, 2000-2005 sequences 
      used codons more similar to those present in highly expressed pig genes compared 
      to the 1991-1995 set. Twenty four sites of positive selection and 20 sites of 
      negative selection were found in GP5, most of them in transmembrane regions. 
      Additional glycosylation in N37 of GP5 was common in 2000-2005 but some sequences 
      lack a glycosylation site in N46. The hypothetical GP5 was only 88.1% similar to 
      LV and was less hydrophobic. Taking together these results suggest that PRRSV is 
      still adapting to pig cells.
FAU - Mateu, E
AU  - Mateu E
AD  - Departament de Sanitat i Anatomia Animals, Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain. enric.mateu@uab.es
FAU - Diaz, I
AU  - Diaz I
FAU - Darwich, L
AU  - Darwich L
FAU - Casal, J
AU  - Casal J
FAU - Martin, M
AU  - Martin M
FAU - Pujols, J
AU  - Pujols J
LA  - eng
SI  - GENBANK/DQ009625
SI  - GENBANK/DQ009626
SI  - GENBANK/DQ009627
SI  - GENBANK/DQ009628
SI  - GENBANK/DQ009629
SI  - GENBANK/DQ009630
SI  - GENBANK/DQ009631
SI  - GENBANK/DQ009632
SI  - GENBANK/DQ009633
SI  - GENBANK/DQ009634
SI  - GENBANK/DQ009635
SI  - GENBANK/DQ009636
SI  - GENBANK/DQ009637
SI  - GENBANK/DQ009638
SI  - GENBANK/DQ009639
SI  - GENBANK/DQ009640
SI  - GENBANK/DQ009641
SI  - GENBANK/DQ009642
SI  - GENBANK/DQ009643
SI  - GENBANK/DQ009644
SI  - GENBANK/DQ009645
SI  - GENBANK/DQ009646
PT  - Journal Article
DEP - 20051102
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Codon)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Adaptation, Biological
MH  - Amino Acid Sequence
MH  - Codon/genetics
MH  - DNA, Viral/chemistry/genetics
MH  - *Evolution, Molecular
MH  - Glycosylation
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phylogeny
MH  - Polymorphism, Genetic
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - Protein Structure, Tertiary/genetics
MH  - Selection, Genetic
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Spain
MH  - Viral Envelope Proteins
MH  - Viral Proteins/*genetics
PMC - PMC7172362
EDAT- 2005/11/05 09:00
MHDA- 2006/05/17 09:00
PMCR- 2005/11/02
CRDT- 2005/11/05 09:00
PHST- 2005/07/11 00:00 [received]
PHST- 2005/09/21 00:00 [revised]
PHST- 2005/09/21 00:00 [accepted]
PHST- 2005/11/05 09:00 [pubmed]
PHST- 2006/05/17 09:00 [medline]
PHST- 2005/11/05 09:00 [entrez]
PHST- 2005/11/02 00:00 [pmc-release]
AID - S0168-1702(05)00292-3 [pii]
AID - 10.1016/j.virusres.2005.09.008 [doi]
PST - ppublish
SO  - Virus Res. 2006 Feb;115(2):198-206. doi: 10.1016/j.virusres.2005.09.008. Epub 
      2005 Nov 2.