PMID- 16269710
OWN - NLM
STAT- MEDLINE
DCOM- 20051220
LR  - 20190108
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 11
DP  - 2005 Nov
TI  - Links between plant and rhizoplane bacterial communities in grassland soils, 
      characterized using molecular techniques.
PG  - 6784-92
AB  - Molecular analysis of grassland rhizosphere soil has demonstrated complex and 
      diverse bacterial communities, with resultant difficulties in detecting links 
      between plant and bacterial communities. These studies have, however, analyzed 
      "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most 
      closely with plants through utilization of root exudates. The aim of this study 
      was to test the hypothesis that plant species was a major driver for bacterial 
      rhizoplane community composition on individual plant roots. DNA extracted from 
      individual roots was used to determine plant identity, by analysis of the plastid 
      tRNA leucine (trnL) UAA gene intron, and plant-related bacterial communities. 
      Bacterial communities were characterized by analysis of PCR-amplified 16S rRNA 
      genes using two fingerprinting methods: terminal restriction fragment length 
      polymorphisms (T-RFLP) and denaturing gradient gel electrophoresis (DGGE). Links 
      between plant and bacterial rhizoplane communities could not be detected by 
      visual examination of T-RFLP patterns or DGGE banding profiles. Statistical 
      analysis of fingerprint patterns did not reveal a relationship between bacterial 
      community composition and plant species but did demonstrate an influence of plant 
      community composition. The data also indicated that topography and other, 
      uncharacterized, environmental factors are important in driving bacterial 
      community composition in grassland soils. T-RFLP had greater potential resolving 
      power than DGGE, but findings from the two methods were not significantly 
      different.
FAU - Nunan, Naoise
AU  - Nunan N
AD  - Biomathematics and Statistics Scotland, Scottish Crop Research Institute, Dundee, 
      United Kingdom.
FAU - Daniell, Timothy J
AU  - Daniell TJ
FAU - Singh, Brajesh K
AU  - Singh BK
FAU - Papert, Artemis
AU  - Papert A
FAU - McNicol, James W
AU  - McNicol JW
FAU - Prosser, James I
AU  - Prosser JI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, Transfer, Leu)
RN  - 0 (Soil)
SB  - IM
MH  - Bacteria/*classification/genetics/growth & development
MH  - DNA, Bacterial/analysis
MH  - *Ecosystem
MH  - Electrophoresis/methods
MH  - Plant Roots/*microbiology
MH  - Poaceae/*classification/genetics/*growth & development/microbiology
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Plant/analysis/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - RNA, Transfer, Leu/analysis/*genetics
MH  - Soil/analysis
MH  - *Soil Microbiology
MH  - Species Specificity
PMC - PMC1287736
EDAT- 2005/11/05 09:00
MHDA- 2005/12/21 09:00
PMCR- 2005/11/01
CRDT- 2005/11/05 09:00
PHST- 2005/11/05 09:00 [pubmed]
PHST- 2005/12/21 09:00 [medline]
PHST- 2005/11/05 09:00 [entrez]
PHST- 2005/11/01 00:00 [pmc-release]
AID - 71/11/6784 [pii]
AID - 2463-04 [pii]
AID - 10.1128/AEM.71.11.6784-6792.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 Nov;71(11):6784-92. doi: 
      10.1128/AEM.71.11.6784-6792.2005.