PMID- 16269781
OWN - NLM
STAT- MEDLINE
DCOM- 20051220
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 11
DP  - 2005 Nov
TI  - Anaerobic microbial communities in Lake Pavin, a unique meromictic lake in 
      France.
PG  - 7389-400
AB  - The Bacteria and Archaea from the meromictic Lake Pavin were analyzed in samples 
      collected along a vertical profile in the anoxic monimolimnion and were compared 
      to those in samples from the oxic mixolimnion. Nine targeted 16S rRNA 
      oligonucleotide probes were used to assess the distribution of Bacteria and 
      Archaea and to investigate the in situ occurrence of sulfate-reducing bacteria 
      and methane-producing Archaea involved in the terminal steps of the anaerobic 
      degradation of organic material. The diversity of the complex microbial 
      communities was assessed from the 16S rRNA polymorphisms present in terminal 
      restriction fragment (TRF) depth patterns. The densities of the microbial 
      community increased in the anoxic layer, and Archaea detected with probe ARCH915 
      represented the largest microbial group in the water column, with a mean 
      Archaea/Eubacteria ratio of 1.5. Terminal restriction fragment length 
      polymorphism (TRFLP) analysis revealed an elevated archaeal and bacterial 
      phylotype richness in anoxic bottom-water samples. The structure of the Archaea 
      community remained rather homogeneous, while TRFLP patterns for the eubacterial 
      community revealed a heterogeneous distribution of eubacterial TRFs.
FAU - Lehours, Anne-C
AU  - Lehours AC
AD  - Laboratoire de Biologie des Protistes, UMR CNRS 6023, 24 avenue des Landais, 
      63177 Aubiere Cedex, France. A-Catherine.LEHOURS@univ-bpclermont.fr
FAU - Bardot, Corinne
AU  - Bardot C
FAU - Thenot, Aurelie
AU  - Thenot A
FAU - Debroas, Didier
AU  - Debroas D
FAU - Fonty, Gerard
AU  - Fonty G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Anaerobiosis
MH  - Archaea/*classification/genetics/growth & development
MH  - DNA, Archaeal/analysis
MH  - DNA, Bacterial/analysis
MH  - *Ecosystem
MH  - France
MH  - Fresh Water/*microbiology
MH  - Methane/metabolism
MH  - Oligonucleotide Probes/genetics
MH  - Oxygen/pharmacology
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sulfur-Reducing Bacteria/*classification/genetics/growth & development
PMC - PMC1287608
EDAT- 2005/11/05 09:00
MHDA- 2005/12/21 09:00
PMCR- 2005/11/01
CRDT- 2005/11/05 09:00
PHST- 2005/11/05 09:00 [pubmed]
PHST- 2005/12/21 09:00 [medline]
PHST- 2005/11/05 09:00 [entrez]
PHST- 2005/11/01 00:00 [pmc-release]
AID - 71/11/7389 [pii]
AID - 0029-05 [pii]
AID - 10.1128/AEM.71.11.7389-7400.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 Nov;71(11):7389-400. doi: 
      10.1128/AEM.71.11.7389-7400.2005.